Sedimentation, electrophoresis

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$Microsoft PowerPoint - Intermedier filamentumok [Kompatibilis m\363d]$

F frict = fv Fcentrifug = 2 mrω Fupthrust m = 2 m 2 2⎛ ρm ⎞ fv = mrω − ρm rω = mrω ⎜1− ⎟ ρ ⎝ ρ ⎠ but a = rω 2 2 ρ rω ρ m Upon sedimentation the velocity of the particle increases while going further from axis! (v ≠ constant)
Theodore Svedberg Swedish chemist (1884-1971) Nobel-prize in 1926 S = v 2 rω to measure ⎛ ρk ⎞ m⎜1− ⎟ = ⎝ ρ ⎠ f to calculate S = sedimentation constant Unit: 1 Sv = 10 -13 s <strong>Sedimentation</strong> velocity for unit acceleration shape factor! Relationship between shape factor (f) and diffusion constant (D): k ⋅T D R ⋅T N D To determine the mass sedimentation methods must be combined with diffusion measurements. f = = A ⋅ kkkk:::: Boltzmann constant R: R: R: R: universal gas constant NNNN : Avogadro-number. = ∙ ∙ ∙ ∙ ( − )
Page 1 and 2: Sedimentation, electrophoresis Biop
Page 3 and 4: F upthrust = ρ mVg ρρ km m,
Page 5: What is centrifugal force? No accel
Page 10 and 11: Velocity boundary sedimentation met
Page 12 and 13: Energy energia E 2 E 1 to calculate
Page 14 and 15: F centrifug = 3. Density gradient m
Page 16 and 17: Electrophoresis Ionic double layer
Page 18: Agarose gel electrophoresis
Page 21 and 22: SDS polyacrylamide gel electrophore
Page 23 and 24: Native PAGE
Page 25 and 26: 2D electrophoresis
Page 27: iotech.szbk.u-szeged.hu/KK_Jegyzet/

Sedimentation, electrophoresis

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