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Gram-negative facultative anaerobic rods

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Eosin-methylene blue agar<br />

Selective and differentiating medium:<br />

- Eosin and methylene blue inhibit the growth of<br />

<strong>Gram</strong>-positive bacteria<br />

- Anionactive detergent (eg. Na-laurylsulphate)<br />

inhibit the swarming of proteae<br />

- Contains lactose. Bacteria degrading lactose<br />

produce acid that precipitates eosin that will be<br />

stained by methylene blue → the colonies of<br />

lactose positive bacteria are blue those of the<br />

lactose <strong>negative</strong>s are pink or grayish<br />

- Advantage: acid will be formed just under<br />

colonies<br />

2006. 03. 06.

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