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PATHOLOGY. VII. Issues <strong>in</strong> immunohistochemistry<br />
* KIT (CD117) detectable <strong>in</strong> ~ 90% of GIST;<br />
* technical considerations:<br />
- source of Ab (for standadization);<br />
- use of epitope retrieval;<br />
* <strong>in</strong>terpretive considerations:<br />
- # sta<strong>in</strong><strong>in</strong>g pattern;<br />
- # sta<strong>in</strong><strong>in</strong>g <strong>in</strong>tensity, often with<strong>in</strong> same lesion;<br />
- KIT expressed by other tumors;<br />
* not all <strong>GISTs</strong> express KIT;<br />
* evaluation for k<strong>in</strong>ase mutations (for confirm<strong>in</strong>g<br />
diagnosis);<br />
* sta<strong>in</strong><strong>in</strong>g <strong>in</strong>tensity not predict<strong>in</strong>g imat<strong>in</strong>ib mesylate<br />
sensitivity<br />
Medeiros et al Am J Surg Pathol. 2004;28:889.<br />
Miett<strong>in</strong>en et al Mod Pathol. 2000;13:1134.<br />
Fletcher et al Hum Pathol. 2002;33:459.
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