CHARACTERIZATION OF PARASITE ANTIGENS ... - PAHO/WHO

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- . 1 10 fractions were isolated by DEAE cellulose chromatography. The fractions were all glycoprotein complexes containing glucose and ribose with different amino acids. The two fractions with the highest carbohydrate content were the most antigenic. Working with larvae of T. spiralis, Kent (62) isolated four antigenic glycoprotein fractions by column chromatography. The antigens of T. spiralis have been studied extensively. Witebsky et al. (128) prepared a CF antigen by heating an extract of larvae in a boiling water bath. Melcher (79) prepared acid soluble and insoluble fractions from an extract of delipi-. dizing lyophilized larvae. Labzoffsky et al. (70) isolated eight fractions from larvae with a pyrimidine extraction. Chemical analysis revealed glycoprotein and carbohydrate characteristics. The antigens reacted differently to circu- lating antibody in the serum of rabbits at different stages of the infection. Sleeman and Muschel (106) fractionated the larval antigen into ethanol-soluble and ethanol-insoluble components. Of interest is the fact that Witebsky used his boiled antigen at two dilutions (1:2 and 1:20) for maxium sensitivity in the CF test. These dilutions corresponded to Sleeman and Muchell's ethanol-insoluble and ethanol-soluble fractions with regard to serologic reactivity. The ethanol-soluble antigen absorbed S. typhosa agglutinins present in the sera of trichinella patients. b~~~~~~~~~~~~~~~~~l
Chemical analysis for these antigens (105) revealed that the ethanol-soluble antigen was a glycoprotein (75% protein and 15% carbohydrate) with its carbohydrate portion composed of only glucose units. In light of Weiner and Neely's (125) studies, one would expect to find some rhamnose as well. Attempts to split off the protein or the carbohydrate moiety resulted in denaturation of the antigen. The ethanol-insoluble antigen was a nucleoprotein with the nucleic acid portion being DNA (60%) and protein (14%). The protein moeity was the antigenic substance in the complex. Tanner and Gregory (121) analyzed extracts of larvae of T. spiralis by immunoelectrophoresis. Tanner (119) found that while most of the trichina antigens were proteins that could be precipitated with 5% trichloracetic acid, the major antigen was not precipitable by 5% trichloracetic acid and contained some polysaccharides. This component had an isoelectric point similar to human gamma globulin and was heat labile. Enzyme susceptibility studies(l20) identified this major antigen to be a mucoprotein. The specific enzyme employed to degrade this antigen was mucoprotenase lysozyme. The antigens of Echinococcus species (hydatid fluid, scolices, and membranes) have been popular materials for antigenic analysis. J0 11 r
Page 1 and 2: CHARACTERIZATION OF PARASITE ANTIGE
Page 3 and 4: The biological and immunological ac
Page 5 and 6: e Nonspecific, Cross-reacting Paras
Page 7 and 8: to mammalian glycogen composed of 1
Page 9 and 10: * Host antigens present in the para
Page 11: A lipoprotein was isolated from Fas
Page 15 and 16: _W - 13 e in hydatid fluid as a glo
Page 17 and 18: 5-6 antigen-antibody complexes by c
Page 19 and 20: Qs analysis seven specific adult wo
Page 21 and 22: * components were identified, and w
Page 23 and 24: .Q were visualized by their antiser
Page 25 and 26: *e The chemical composition of an A
Page 27 and 28: e J1 Differences between all specie
Page 29 and 30: REFERENCES 1. Agosin, M. Broquimica
Page 31 and 32: ( 3 ( " 15. Von Brand, T. Old and n
Page 33 and 34: 28. Damian, R. T. Common antigens b
Page 35 and 36: 44. Hariri, M. N., C. W. Sch .je, a
Page 37 and 38: 60. Kent, N. H. Isolation of specif
Page 39 and 40: 77. Magath, T. B. The antigen of ec
Page 41 and 42: 92. Pellegrino, J., E. Paulini, J.
Page 43 and 44: 106. Sleeman, H. K., and L. H. Musc
Page 45 and 46: 122. Tarrant, C. J., E. H. Fife, an
Page 47 and 48: . Plate A Figure 1. Figure 2. Figur
Page 49 and 50: * Plate B - An immunoelectrophoreti
Page 51 and 52: . Plate C Figure 9. Figure 10. Figu
Page 53: I ~ ~ ~ ~ ~~_ .- 1-' i.~~~~~~~~~i~

CHARACTERIZATION OF PARASITE ANTIGENS ... - PAHO/WHO

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