CHARACTERIZATION OF PARASITE ANTIGENS ... - PAHO/WHO

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We chose hydatid fluid of E. granulosus early in our antigenic
analysis work because it was a biological fluid with a strong
antigenicity and bore a striking resemblance to paper electro-
phoretic patterns obtained with serum of the host (42). We
have, to date, identified 19 antigenic components in sheep
hydatid fluid (24). At least two polysaccharides have been
described (2, 64) as have end products of carbohydrates and
protein metabolism (1).
Polysaccharide antigens have been isolated from laminated
membrane and probably germinal membrane by a number of workers.
Agosin et al. (2) separated the polysaccharide antigens in two
components by electrophoresis and found a mobility similar to
that of glycogen. The second contained glucosamine and galactose.
Kilejian et al. (64) isolated a mucopolysaccharide. Working in
our laboratory she was able to coat latex particles with this
antigen and found it to be reactive with sera from immunized
animals but not with sera from infection. Magath (77) reported
that an echinococcus antigen xa- active in the CF test moved
like a gamma globulin by immunoelectrophoresis (I. E.) Paulete-
Venrell et al. (90) reported that their antigen moved in an
immunoelectrophoretic field like beta and gamma globulins.
Harari et al. (44) identified an immunologically active component