CHARACTERIZATION OF PARASITE ANTIGENS ... - PAHO/WHO

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anodically and reacted with human antisera; peak 2, 22 mm; and peak 3, 14 mm. The latter two components were not active in diagnostic tests. Caetano da Silva and Guimaraes Feiri (17) found 1-4 precipitin bands in the serum of 78% of patients with hepatosplenic schistosomiasis versus one band in only 38% of patients with hepatointestinal schistosomiasis. In a second paper (18) these authors published data on a reverse immunoelectrophoretic technique. Serum was fractionated in an electrical field and developed with antigen of S. mansoni. Precipitin bands in the IgM and IgG position were visualized. Kent (63) analyzed adult and cercarial extracts in terms of protein, carbohydrate, and lipid. He was able to show that a considerable portion of the lyophilized antigen is water soluble. Ten protein systems in adult and eight in cercariae were detected by immunoelectrophosesis. One cross-reacting anti gen with T. spiralis was demonstrated. Biguet et al. (10) were able to demonstrate eight proteins, five glycoproteins, and one lipoprotein in adult extracts of S. mansoni. Stahl et al. (116) were able to demonstrate antibodies to egg antigen-antibody complexes. In our work (53) we were able to demonstrate by agar gel 16
Qs analysis seven specific adult worm, three cercarial, and five egg antige-ns. In all, 25 different antigenic bands were demonstrated by Ouchterlony gel diffusion analysis. Analysis of antigens prepared by various methods such as delipidization with anhydrous ether (Chaffee antigen), petroleum ether (Melcher antigen), and crude extract were made. In these extracts, five of seven adult antigens were shared in common. Immunoelectrophoretic studies with antisera prepared in rabbits showed the complexity of our schistosome extracts. An extract of adult S. mansoni containing 0.87 mg N/ml was developed/\(Fig. 1) with a serum prepared against the crude antigen. In each figure the numbers designated to a line of precipition are based on order of appear- ance and not antigenic relationship. In Figure 2 an extract of adult worms prepared by the Melcher (79) technique was developed with the same serum. A total of at least 16 components in the crude extract (Fig. 1) and 11 components in the Melcher extract (Fig. 2) were identified. A delipidized cercarial extract(prepared by extraction with anhydroua ether) developed against thesamq antiserum revealed at least 18 components (Fig. 3). The same antigen developed with an anti-Chaffee adult serum shows a slightly different configuration (Fig. 4). Absorption studies indicated that all but perhaps one band are shared by the cercariae and the adult. J 17
Page 1 and 2: CHARACTERIZATION OF PARASITE ANTIGE
Page 3 and 4: The biological and immunological ac
Page 5 and 6: e Nonspecific, Cross-reacting Paras
Page 7 and 8: to mammalian glycogen composed of 1
Page 9 and 10: * Host antigens present in the para
Page 11 and 12: A lipoprotein was isolated from Fas
Page 13 and 14: Chemical analysis for these antigen
Page 15 and 16: _W - 13 e in hydatid fluid as a glo
Page 17: 5-6 antigen-antibody complexes by c
Page 21 and 22: * components were identified, and w
Page 23 and 24: .Q were visualized by their antiser
Page 25 and 26: *e The chemical composition of an A
Page 27 and 28: e J1 Differences between all specie
Page 29 and 30: REFERENCES 1. Agosin, M. Broquimica
Page 31 and 32: ( 3 ( " 15. Von Brand, T. Old and n
Page 33 and 34: 28. Damian, R. T. Common antigens b
Page 35 and 36: 44. Hariri, M. N., C. W. Sch .je, a
Page 37 and 38: 60. Kent, N. H. Isolation of specif
Page 39 and 40: 77. Magath, T. B. The antigen of ec
Page 41 and 42: 92. Pellegrino, J., E. Paulini, J.
Page 43 and 44: 106. Sleeman, H. K., and L. H. Musc
Page 45 and 46: 122. Tarrant, C. J., E. H. Fife, an
Page 47 and 48: . Plate A Figure 1. Figure 2. Figur
Page 49 and 50: * Plate B - An immunoelectrophoreti
Page 51 and 52: . Plate C Figure 9. Figure 10. Figu
Page 53: I ~ ~ ~ ~ ~~_ .- 1-' i.~~~~~~~~~i~

CHARACTERIZATION OF PARASITE ANTIGENS ... - PAHO/WHO

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