CHARACTERIZATION OF PARASITE ANTIGENS ... - PAHO/WHO

Note that after three days of incubation, common antigenic com-
ponents joined and coalesced. In Figure 8 an infected rabbit
serum (N26) and a rabbit antiserum (crude D) were used to develop
the reaction to detect common components in these", Only two or
three antigen-antibody bands were shared. In Figure 9 a human
diagnostic serum (1401) and an infected rabbit serum (N26) were
used to develop the antigenic pattern. Note that only band #4
and #7 are common. In Figure, 10 note the similarity in pattern
between the infected rabbit serum above (P20) and an immune
rabbit serum (LyS) prepared against metabolic secretions of
larve (LXS antigen). In Figure 11 the LXS antiserum is compared
to the crude larvae antiserum with very little evidence for anti-
genic sharing of components. In Figure 12 the LXS antiserum is
compared to a humana The antigenic complexity of a delipidized
extract of larvae of T. spiralis as revealed by this type of
analysis is very great. In all of the reactions, only a few
components are clearly shared - the remainder may be different.
Agar gel analysis of Ascaris tissues and extracts were
reported by Kagan (48), Kagan et al. (56), Soulsby (109), and
Huntley and Moreland (46). Tormo and Chordi (123) prepared
polysaccharide and protein extracts of A. suum for analysis by
immunoelectrophoresis. A total of 20 antigenic components
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20