CHARACTERIZATION OF PARASITE ANTIGENS ... - PAHO/WHO

Q
An immunoelectrophoretic analysis of F. hepatica antigen
by Biguet et al. (11) revealed seven protein fractions, two
glycoproteins, and six lipoproteins. Of 15 fractions visualized
with rabbit antisera, five were specific. Szaflarski et al. (117)
attempted to characterize an antigenic mucoprotein prepared with
sulphosalicylic acid using papain and rivanol without success.
Capron et al. (21) identified ,Cprotein substarnce in extracts of
F. hepatica as well in a number of other helminth parasites (12).
Tanner and Gregory (121) showed in their gel diffusion
studies on extracts of larvae T. spiralis that of the 11 bands
they identified individual rabbits developed antibodies to only
some of these components. They also compared the crude extract
of larvae and a Melcher type larval antigen. In most instances
they found the differences between a crude buffered saline extract
and antigens prepared by alkaline and acid extraction after delipi-
dization (Melcher type) were quantitative and not qualitative in
in nature. Dymowska et al. (35) fractionated larvae of T. spiralis
on a starch block and assayed 26 protein fractions. Among these
fractions 9-14 proved to be serologically active. They contained
acid phosphatase and hyaluronidase. The antigehic structure of
T. spiralis was analyzed in detail by Biguet et al. (14). Wíth
antisera produced by immunization in rabbits, 19 antigenic
18