Gradient Master - Wolf Laboratories
Gradient Master - Wolf Laboratories
Gradient Master - Wolf Laboratories
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4.6.c. Resolving small protein complexes:<br />
The figure below from Peter Sorger’s lab, then at MIT, shows the purification of a small protein<br />
complex (210 kD heterodecamer; 7.4S) on glycerol gradients using the <strong>Gradient</strong> <strong>Master</strong> and Piston<br />
Fractionator. This rate zonal gradient clearly outperformed columns in the purification, both in peak<br />
width and r2 values for the marker proteins on two separate runs.<br />
(Miranda J.J., De Wulf, P., Sorger, P.K., Harrison, S.C. 2005. The yeast DASH complex forms closed<br />
rings on microtubules Nat Struct Mol Biol. Feb;12(2):138-43.)<br />
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