(LATO) IN LOWERED SEAWATER pH - Central Visayas Campus ...

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3.4.3.2 Spectrophotometric Determination The supernatant for each treatment was analyzed for the determination of the chlorophyll a content using a UV-Vis spectrophotometer. The chlorophyll a concentration was determined by measuring the absorbance (optical density - OD) of the extract at wavelengths 645 nanometer and 662 nanometer. The resulting absorbance measurements were then applied to a standard equation (ESS Method 150.1: Chlorophyll-Spectrophotometric). The formula below was used to calculate the amount of chlorophyll a: C = (11.75) (A662) – (2.350) (A645) where C = Chlorophyll a concentration, 3.5 Statistical Analysis A662 = absorbance at 662 nanometer A645 = absorbance at 645 nanometer. Treatment effects on the weight of the species were statistically tested for significance at the 5% level using t-test (Kurihara and Ishimatsu 2008). The initial and final weights of the species for the three trials were used and compared statistically. The program used was Past software in computing the t-test. 16
CHAPTER IV RESULTS AND DISCUSSION 4.1 Average Weight of Caulerpa lentillifera at Different pH Samples of Caulerpa lentillifera for four different pH levels were weighed before and after treating them at varying pH. These were then calculated and the average weight for the species in each tank were determined. Figure 2. Mass (in grams) of C. lentillifera at different pH Figure 1 shows the result of the average weight of the species. The graph of pH ranging from 7.7 to 8.3 has showed an increase in the mass while the graph of pH ranging from 5.7 to 6.3, pH 3.7 to 4.3 and pH 2.7 to 3.3 showed a decrease in the mass. As the pH decreases compared to normal seawater pH, the final mass of C. lentillifera also decreases. This coincides to the fact that C. lentillifera can thrive on the normal seawater pH which is at around pH 8 (Battistoni et al., 2007). Using t-test, the computed t-values of the trials with pH 8.0±0.3, pH 6.0±0.3, pH 4.0±0.3 and pH 3.0±0.3 have values that are lesser than the significance level of 0.05.
Page 1 and 2: GROWTH PERFORMANCE OF Caulerpa lent
Page 3 and 4: BIOGRAPHICAL DATA Researcher : Chri
Page 5 and 6: ACKNOWLEDGEMENT The researchers wou
Page 7 and 8: TABLE OF CONTENTS Title PAGE TITLE
Page 9 and 10: LIST OF TABLES TABLE PAGE 1 t-Test
Page 11 and 12: 1.1 Background of the Study CHAPTER
Page 13 and 14: 1.3 Significance of the Study This
Page 15 and 16: 2.1 Ocean Acidification CHAPTER II
Page 17 and 18: 2.2 Ocean Acidification’s Effects
Page 19 and 20: and Ratana-arporn in 2006 concluded
Page 21 and 22: Lowering pH resulted in a decrease
Page 23 and 24: 3.1 Research Design CHAPTER III MET
Page 25: where K = growth rate, N1 = initial
Page 29 and 30: These studies correlate with the re
Page 31 and 32: 6.0±0.3 changed from green to pale
Page 33 and 34: 5.1 Conclusion CHAPTER V CONCLUSION
Page 35 and 36: BIBLIOGRAPHY Apiratikul, R. & Pavas
Page 37 and 38: Jenkins, A. (2009). A Closer Look a
Page 39 and 40: APPENDIX A RAW DATA OF THE INITIAL
Page 41 and 42: APPENDIX B RAW DATA OF THE ABSORBAN
Page 43: APPENDIX D PHOTOS Figure D.1 Fresh

(LATO) IN LOWERED SEAWATER pH - Central Visayas Campus ...

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