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(LATO) IN LOWERED SEAWATER pH - Central Visayas Campus ...

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<strong>pH</strong> necessary for cell function. In addition, Hinga (2002) also concluded that the low<br />

concentrations of free CO2 may as well be the mechanism for reduced growth rate at<br />

low <strong>pH</strong>. The <strong>pH</strong> also affects the rates of primary production of marine algae.<br />

Accordingly, their growth rates change significantly with <strong>pH</strong> changes near the<br />

equilibrium <strong>pH</strong>.<br />

The results of the study showed that the growth rate of the species was<br />

reduced in lowered <strong>pH</strong>, which also coincided with the results on the study by Hinga<br />

(2002). At <strong>pH</strong> 8.0±0.3, the species are under their normal environment, enabling<br />

them to perform its activities well.<br />

4.3 Color Changes of the Samples<br />

The samples treated at the acidic <strong>pH</strong> of 3.0±0.3 and 4.0±0.3 showed a change<br />

in color, most specifically from green to pale white. Also, 100% of the sample at these<br />

<strong>pH</strong> ranges did not survive the experimental period of 7 days. On the other hand, only<br />

about 20% of the species treated at <strong>pH</strong> 6.0±0.3 showed a change in color (see<br />

Appendix D). The species treated at <strong>pH</strong> 8.0±0.3 had shown an even darker green<br />

color, implying an increase in growth (Atkinson and Marubini, 1999).<br />

Chlorophyll is one of the most important part of a plant or algae. Chlorophyll<br />

presence is indicated by a green-colored characteristic in plants mainly because<br />

chlorophyll is green. The greener the algae, the more amount of chlorophyll it<br />

contains (Iglesias-Prieto et al., 1992).<br />

The results in the study by Iglesias-Prieto et al. in 1992 coincides with the<br />

observations in the experiment conducted. The samples at <strong>pH</strong> 8.0±0.3 showed a<br />

darker green color (see Appendix D), implying a higher chlorophyll a content<br />

compared to the samples treated in lowered seawater <strong>pH</strong>. The samples under <strong>pH</strong><br />

20

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