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A survey of freshwater fishes and their macroparasites in the ... - NINA

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13<br />

<strong>NINA</strong> Report 635<br />

We have also used a resampl<strong>in</strong>g procedure to illustrate <strong>the</strong> relationship between host sample<br />

size <strong>and</strong> <strong>the</strong> number <strong>of</strong> parasite taxa recorded <strong>in</strong> <strong>the</strong> sample. For any sample (e.g. <strong>the</strong> number<br />

<strong>of</strong> fish hosts, ns, dissected one year at a given location) we draw, at r<strong>and</strong>om without replacement,<br />

a sub-sample <strong>of</strong> n fish (n=1, 2, …, ns) <strong>and</strong> count <strong>the</strong> number <strong>of</strong> parasite taxa belong<strong>in</strong>g<br />

to this sub-sample. This is repeated 1000 times for each value <strong>of</strong> n. We <strong>the</strong>reby obta<strong>in</strong> a curve<br />

describ<strong>in</strong>g <strong>the</strong> relationship between host sample size <strong>and</strong> number <strong>of</strong> parasite taxa (average <strong>of</strong><br />

<strong>the</strong> 1000 simulations), which is similar to a rarefaction curve (S<strong>and</strong>ers 1968). In addition, <strong>the</strong><br />

simulations provide an <strong>in</strong>dication <strong>of</strong> <strong>the</strong> uncerta<strong>in</strong>ty <strong>in</strong> <strong>the</strong> sample size – parasite taxa relationship.<br />

No. parasite species<br />

0 5 10 15<br />

Locality 7 - 2001 - Dry season<br />

0 20 40 60 80 100<br />

No. resampled host <strong>in</strong>dividuals<br />

Figure 2 Example <strong>of</strong> <strong>the</strong> relationship<br />

between host number<br />

<strong>and</strong> parasite species number<br />

based on <strong>the</strong> resampl<strong>in</strong>g procedure<br />

described <strong>in</strong> <strong>the</strong> text.<br />

The dotted l<strong>in</strong>es give approximate<br />

95% confidence limits,<br />

but <strong>the</strong> upper limit from ca. 70<br />

resampled hosts is restricted<br />

to <strong>the</strong> total number <strong>of</strong> observed<br />

parasite species (15)<br />

<strong>and</strong> should <strong>the</strong>refore not be<br />

applied.<br />

Rio Sapoa (locality<br />

2; 205 m a.s.l.).

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