sbornik_s_2011.pdf

XI. Woorkshop
of Physsical
Chemists and a Electrochemmists´11
skeleetal
muscullature
and intense paiin.
Death occurs o after r one hourrs
through respiratoryy
parallysis
or heaart
failure [3 3, 9-11].
However, , some studies
have demonstra ated also phenolic
coonstituents,
especiallyy
flavoonol
and hyydroxyphen
netyl glycoosides
with antioxidan nt propertiees
[12-14]. Flavonoidss
havee
been fouund
in some
subspeecies
of Aconitum A napellus n – A. napel llus subsp. .
neommontanum
(Wulfen) ) Bayer (flowers - querceti in 7-O-(66-trans-caff
feoyl)-beta-
glucoopyranosyll-(1,3)-alpha-rhamnoppyranoside-3-O-beta-g
glucopyrannoside,
kaem mpferol7- O-(66-trans-cafffeoyl)-beta-glucopyrannosyl-(1,3)-
-alpha-rham mnopyranooside-3-O-b
beta
glucoopyranosidde
and kae empferol 77-O-(6-tran
ns-p-coumaroyl)-beta-glucopyran
nosyl-(1,3)-
alphaa-rhamnoppyranoside
3-O-beta-gglucopyrano
oside together t
with beta-3,4-
dihyydroxyphennethyl
beta a-glucopyraanoside)
an nd A. nape ellus subspp.
tauricum m (Wulfen) )
Bayeer,
species distributed
in Easteern
Alps and a southe ern Carpatthians
(3-O O-(6-trans-
caffeeoyl)-beta-gglucopyranosyl-(1,2)-bbeta-glucop
pyranoside-7-O-alphaa-rhamnopy
yranoside,
kaemmpferol
3-O-(6-trans
s-caffeoyl)-bbeta-glucop
pyranosyl-( (1,2)-beta-gglucopyran
noside-7-O-
alphaa
-rhamnoopyranosid
de, quercettin
3-O-(6 6-trans-p-co oumaroyl)-beta-gluco
opyranosyl-
(1,2) -beta-glucoopyranoside
e-7-O-alphha-rhamnop
pyranoside aand
beta-3,4-
dihyydroxyphennethyl
beta-glucopyrannoside)
[15 5, 16]. Flav vonoids havve
been fou und also inn
other
members of Aconitu um genus [117].
Fig.
1: The most
import tant diterppene
alkalo oids of Aco onitum nap apellus aggr regate. 1 -
napellinne,
Fig. 2: aconitinne
- (R
hypacon
1 = -
nitine (R1 -CH2CH3, R
= CH3, R2 R
= H
2 = -OH), mesakonit tine (R
H)
1 = --CH3,
R2 = -OH) andd
Plants of Aconitum genus are uused
in tra aditional Ch hinese meddicine
after processingg
to ddecompose
toxic alka aloids to the less toxic t deriv vatives andd
for prep paration off
hommeopathic
prreparations
s [18-20].
2. EXPERIMMENT
Plant maaterial
(roo ots, stems, leaves) was w collecte ed in the botanical garden off
Univversity
of VVeterinary
and a Pharmmaceutical
University U Brno. For tthe
anatom mical study, ,
transsversal,
raddial
and tan ngential hannd-made
se ections wer re used. Foor
the visua alization off
ligniified
plant ttissues,
ethanolic
(50 % %, v/v) mix xture of acid
fuchsinee
and malac chite greenn
(bothh
1%, w/ww;
Sigma-Al ldrich, USAA)
was use ed. Sections s were stainned
for fou ur minutess
and aafter
it wasshed
with acidic a ethannol
(60 %, v/v, v with 37 7% hydrochhloric
acid 0.1 %, v/v, ,
- 25 -
Brnoo