sbornik_s_2011.pdf

XI. Workshop of Physical Chemists and Electrochemists´11 Brno
A B C
O O
O O
OH
O
OH
D E F
O
OH
O
O
OH
OH
O
OH
OH
Fig. 1: The most important chemical constituents of Maclura pomifera. Osajin (A),
pomiferin (B), scandenone (C), auriculasin (D), alvaxanthone (E) and 8prenyltoxiloxanthon
(F)
Family Moraceae consists of shrubs, trees and woody wines with lactifers containing
milky latex. Some species are important for “fruits”, respectively multiple fruits – figs
(Ficus carica), mulberry (Morus spp.), and bread-fruit (Artocarpus faltitis). Members of
genus Ficus are studied because potential pharmacological usage [16, 17]. Some plants are
important as ornamental, widely cultivated plants (Ficus benjamina). Some plants are
poisonous. Antiaris toxicaria, plant native to Australia, Asian countries and some African
countries, such as Uganda or Sudan, is used for hunting. The latex contains cardiac
glycosides, such as antiarin, antiarosides and antiarotoxin [18, 19]. Latex is used as an
arrow poison called upas. Despite above-mentioned facts, knowledge about anatomy of
these plants is still missing. Submitted work is focused on the anatomical study of
vegetative organs Maclura pomifera (Raf.) C.K.Schneid.
2. EXPERIMENT
Plant material (roots, stems, leaves) was collected in the botanical garden of
University of Veterinary and Pharmaceutical University Brno. For the anatomical study,
transversal, radial and tangential hand-made sections were used. For the visualization of
lignified plant tissues, ethanolic (50 %, v/v) mixture of acid fuchsine and malachite green
(both 1%, w/w; Sigma-Aldrich, USA) was used. Sections were stained for four minutes
and after it washed with acidic ethanol (60 %, v/v, with 37% hydrochloric acid 0.1 %, v/v,
Sigma-Aldrich, USA). At once, sections, which were not stained, were prepared for
fluorescence microscopic analysis (Carl Zeiss Axioscop 40, Zeiss, Germany). In addition,
sections were stained by aqueous solution of acridine orange (1 %, w/w, Sigma-Aldrich,
USA).
O
O
O
- 33 -
OH
OH
OH
OH
O
O
OH
O
O
O
O
OH
OH
OH