CHOLERA- Dr. J.J. Kambona [Compatibility Mode]

Investigations
4. Stool culture:
Routine differential media:
A. Triple sugar iron agar:
Gives the non-pathogenic pattern of an acid (yellow) slant,
because of fermentation of sucrose contained in the media.
B. Alkaline enrichment media:
Peptone water (pH 8.5-9.0).
Media containing bile salts e.g. thiosulphate–citrate bilesucrose
agar (pH 8.6). Sucrose fermenting vibrio cholerae
grow as large, smooth, round yellow colonies that stand out
against the blue-green agar.