Life Science - Promega

Life
Science
Catalog
2012
Worldwide Contact List
Section
Contents
Cell Signaling
Nucleic Acids
Vitronectin, Human
Product Size Cat.# Price ($)
Vitronectin, Human 100 μg G5381 576.00
For Research Use Only. Not for Use in Diagnostic Procedures.
Description: Human Vitronectin is purified from plasma. Vitronectin belongs
to the group of structurally and functionally homologous adhesive proteins
(fibrinogen, fibronectin, Von Willebrand factor) that interact with platelets and
the vessel wall in the early stages of blood clotting. When coated on surfaces,
very low concentrations of Vitronectin promote endothelial cell attachment
and induce spreading and migration of cells in a time- and concentrationdependent
fashion.
Activity: When coated onto tissue culture plastic, Vitronectin promotes onehalf
maximal attachment of BALB/3T3 fibroblasts in serum-free medium below
0.1μg/cm 2 . Maximal attachment occurs at approximately 0.2μg/cm 2 .
Storage Conditions: Store at –70°C.
Protocol
Vitronectin, Human Protocol
Western Blue ® Stabilized Substrate for
Alkaline Phosphatase
Part#
9PIG538
Product Size Cat.# Price ($)
Western Blue ® Stabilized Substrate for Alkaline
Phosphatase 100 ml S3841 87.00
For Research Use Only. Not for Use in Diagnostic Procedures.
Description: Western Blue ® Stabilized Substrate for Alkaline Phosphatase
is a stable, ready-to-use substrate for Western blots and immunoscreening.
Western Blue ® Substrate should be used directly and without dilution. It is a
mixture of 5-bromo-4-chloro-3-indolyl-phosphate (BCIP) and nitro blue tetrazolium
(NBT) in a proprietary stabilizing buffer. This liquid substrate deposits a
permanent dark purple stain on membrane sites bearing alkaline phosphatase.
Western Blue ® Substrate is as sensitive as BCIP/NBT reagents.
Features:
• Convenient: Use directly without diluting or mixing.
• Sensitive: Substrate is as sensitive as other BCIP/NBT reagents.
• Stable: Stable for one year at room temperature.
Storage Conditions: Store at 22–25°C.
X-Gal (5-bromo-4-chloro-3-indolyl-β-dgalactopyranoside)
Product Size Conc. Cat.# Price ($)
X-Gal 100mg/2 ml 50 mg/ml V3941 107.00
For Laboratory Use.
Description: X-Gal, in conjunction with IPTG, is used to detect β-galactosidase
activity to differentiate recombinants from nonrecombinants in cloning experiments
using vectors containing the lacZ or lacZ α-peptide gene.
Features:
• Concentration: 50mg/ml in dimethylformamide, 2.0ml/vial.
• Quality Tested: X-Gal is tested for use with the pGEM ® -Z Vectors in a
chromogenicity assay.
Storage Conditions: Store at 4°C or –20°C.
Genomic DNA
Product Size Cat.# Price ($)
Human Genomic DNA: Male 100 μg G1471 224.00
Human Genomic DNA: Female 100 μg G1521 224.00
Human Genomic DNA 100 μg G3041 224.00
Mouse Genomic DNA 100 μg G3091 224.00
G1471, G1521, G3041 For Laboratory Use. G3091 For Research Use Only. Not for Use in
Diagnostic Procedures.
Description: Genomic DNA from selected species are purified, and greater
than 90% of the DNA is longer than 50kb in size as measured by pulsedfield
gel electrophoresis. The DNA is suitable for Southern blot hybridizations,
genomic analysis (including PCR), and genomic library construction. The Mouse
Genomic DNA is isolated from whole blood from disease-free mice. Human
Genomic DNA comes from multiple anonymous donors.
Storage Conditions: Store at 4°C.
Herring Sperm DNA
Product Size Conc. Cat.# Price ($)
Herring Sperm DNA 10 mg 10 µg/µl D1811 67.00
100 mg 10 µg/µl D1815 283.00
500 mg 10 µg/µl D1816 563.00
For Laboratory Use.
Description: Herring Sperm DNA is tested and certified to be free of any
DNase or RNase activity. It is useful as a blocking agent in nucleic acid
hybridization experiments.
Features:
• Quality Tested: Certified to be free of any DNase or RNase activity.
• Multiple Applications: Use as a blocking agent in hybridizations or as
carrier DNA.
• Ready to Use: Provided as a 10mg/ml solution.
• Choose Your Configuration: Learn more about our custom options for
this product at: www.promega.com/myway/
Storage Conditions: Store at –20°C.
Note: Product may be viscous at 4°C. Prior to use, ensure product is at room
temperature (it may be briefly warmed at 37°C) and mixed thoroughly to
ensure homogeneity.
Lambda DNA
Product Size Cat.# Price ($)
Lambda DNA 250 μg D1501 94.00
For Laboratory Use.
Description: λ DNA cl857 Sam7 is isolated from infected E. coli strain
W3350. Restriction enzyme-digested λ DNA (48,502bp) may be used as a
molecular weight size marker in gel analysis of nucleic acids. λ DNA is also a
commonly used substrate in restriction enzyme activity assays. The nucleotide
sequence has been determined.
Features:
• Choose Your Configuration: Learn more about our custom options for
this product at: www.promega.com/myway/
Storage Conditions: Store at –20°C.
Table of
Contents
12
For complete and up-to-date product information visit: www.promega.com/catalog