Life Science - Promega

Cell Signaling
Table of Contents
Promega 2012
Life Science Catalog
1 Biochemicals and Labware 1
2 Cell Health Assays 19
3 Cell Signaling 47
4 Cloning and DNA Markers 71
5 DNA and RNA Purification 105
Table of Contents
ABOUT THE COVER
PERCHED ON A TENDRIL
An extensively ornamented butterfly egg
(Dryasiulia) thrones on a Passiflora tendril. It is a
safe place: tendrils are tough as leather so as to
hold the plant in place while it climbs up tall trees
to reach the lush canopy of the South American
rainforest.
To create the image, the tendril with the egg
was carefully placed on double-adhesive carbon
tape. After that, it was covered with a very thin
layer of gold - just enough to make the sample
electronically conductive. Then, it was put inside
the high-vacuum chamber of the microscope and
slowly scanned with the electron beam, pixel-bypixel
and line-by-line. Since the resulting digital
scan only captures a topographic memory, all the
colors have to be restored subsequently.
© Martin Oeggerli 2010,
kindly supported by PTU.
6 Drug Discovery 135
7 Genetic Identity 147
8 Imaging and Immunological
Detection 161
9 Industrial and Environmental
Monitoring 179
10 Instruments 183
11 Molecular Diagnostics 195
12 PCR 203
13 Protein Expression and Analysis 221
14 Protein Purification and
Interactions 241
15 Reporter Assays and Transfection 257
16 RNA Analysis 283
17 Stem Cell Research 291
18 Vectors 297
19 Index and Legal References 311
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