Life Science - Promega

Life
Science
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2012
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Cell Signaling
Caspase-Glo ® 6 Assay Systems
Product Size Cat.# Price ($)
Caspase-Glo ® 6 Assay 10 ml G0970 648.00
For Laboratory Use.
50 ml G0971 Pls. Enq.
Description: The homogenous Caspase-Glo ® 6 Assay measures caspase-6
acitivity. Caspase-6 is a member of the cysteine aspartic acid-specific protease
family and has a key effector role in the cleavage of specific target proteins
during apoptosis. The Caspase-Glo ® 6 Assay provides a luminogenic substrate,
Z-VEID-aminoluciferin, in a buffer optimized for caspase-6 and luciferase
activity. The addition of a single Caspase-Glo ® 6 Reagent results in cleavage of
the substrate, releasing aminoluciferin, and generating a glow-type luminescent
signal in the presence of Ultra-Glo Recombinant Luciferase. The luminescent
signal is proportional to the amount of caspase-6 activity. The Caspase-Glo ®
6 Assay is designed for use with purified enzyme preparations in multiwell
plate formats, making it ideal for automated high-throughput screening for
caspase-6 activity and inhibitors of caspase-6 activity.
Features:
• Simplified Method: The protocol makes the assay easy to automate.
• Greater Sensitivity: The assay is more sensitive than fluorescence-based
caspase-6 assays and provides excellent signal-to-noise ratios. The assay
is linear over 3 logs of caspase-6 concentration and can detect 0.002U/ml.
• Increased Accuracy: The superior sensitivity over fluorescence-based
caspase assays allows inhibitor studies at concentrations below the K m .
• Faster Results: The maximum signal and sensitivity of the assay is
reached in as little as 30 minutes after reagent addition.
• High-Quality Assay: The assay delivers an excellent Z´-factor value of
0.86 when using 0.1U/ml of caspase-6 for assays in 384-well plates.
• Batch Processing Capability: Stable, continous signall allows plates to
be read over an extended period of time.
Storage Conditions: Store at –20°C.
Protocol
Caspase-Glo ® 6 Assay Technical Bulletin
Part#
TB366
Caspase-Glo ® 8 Assay Systems
Product Size Cat.# Price ($)
Caspase-Glo ® 8 Assay 2.5 ml G8200 180.00
10 ml G8201 602.00
100 ml G8202 Pls. Enq.
For Laboratory Use.
Description: The homogeneous Caspase-Glo ® 8 Assay measures caspase-8
activity using a proluminogenic caspase-8 substrate in a buffer system
optimized for caspase activity, luciferase activity and cell lysis. The addition of a
single Caspase-Glo ® 8 Reagent in an “add-mix-measure” format results in cell
lysis, followed by caspase cleavage of the substrate and generation of a “glowtype”
luminescent signal. The signal generated is proportional to the amount of
caspase activity present. The Caspase-Glo ® Reagent relies on the properties
of a proprietary thermostable luciferase (Ultra-Glo Recombinant Luciferase),
which generates the stable “glow-type” luminescent signal and improves
performance across a wide range of assay conditions.
The system now includes a separate vial of MG-132 protease inhibitor,
which may be used to reduce background, improving the performance of the
Caspase-Glo ® 8 Assay in cell-based applications.
Features:
• Simplify Apoptosis or Caspase Detection: The homogeneous “addmix-read”
protocol makes the assay easy to automate; simply add an equal
volume of reagent to sample volume.
• Use Less Enzyme: Excellent signal-to-noise ratios and superior sensitivity
allow assays to be performed in 96- or 384-well formats.
• Decrease Assay Time: No sample preparation or manipulation required,
and no extended incubation times are necessary as with fluorescent-based
assays. Maximum sensitivity is achieved in as little as 0.5–1 hour.
• Rely on a Performance-Tested Assay: The assay delivers excellent Z´
factor values in cell and purified enzyme models.
• Get More Information: Multiplex with other cell-based assays from
Promega.
• Experience Improved Caspase-8 Selectivity: Caspase-Glo ® 8 Assay
uses a luminogenic substrate containing the LETD sequence, which has
been shown to be selective for caspase-8. The assay includes an optional
proteasome inhibitor (MG-132), which when added to the Caspase-Glo ® 8
Reagent significantly reduces nonspecific background in cell-based assays.
• Automate This Assay: Validated automated methods available at:
www.promega.com/automethods/
• Choose Your Configuration: Learn more about our custom options for
this product at: www.promega.com/myway/
Storage Conditions: Store at –20°C protected from light.
Protocol
Caspase-Glo ® 8 Assay Technical Bulletin
Part#
TB332
Section
Contents
Table of
Contents
28
For complete and up-to-date product information visit: www.promega.com/catalog