Life Science - Promega

Life
Science
Catalog
2012
Worldwide Contact List
Section
Contents
Cell Signaling
NF-κB, Human, Recombinant
Product Size Cat.# Price ($)
rhNF-κB (p50) 50 gsu E3770 402.00
For Research Use Only. Not for Use in Diagnostic Procedures.
Description: NF-κB (p50), Human, Recombinant is expressed in bacteria
from a human cDNA derived from the full-length p105. p50, a processed
product of its p105 precursor, is the first DNA-binding subunit of the NF-κB
transcription factor described. The amino-terminal half of p50 has strong
homology to the proto-oncogene, c-rel, its viral counterpart, v-rel, and the
Drosophila dorsal gene.
p50 forms a transcriptionally active heterodimer with NF-κB subunit p65. The
p50/p65 heterodimer is subject to regulation by physical sequestration in the
cytoplasm by I-κB.
Features:
• Performance-Tested: Tested in gel shift assays.
Storage Conditions: Store at –70°C.
TFIIB, Human, Recombinant
Product Size Cat.# Price ($)
rhTFIIB 50 gsu E3790 402.00
For Research Use Only. Not for Use in Diagnostic Procedures.
Description: rhTFIIB is a general transcription factor involved in formation of
an active complex in vitro capable of specifically initiating RNA synthesis by
RNA polymerase II. An early stage of initiation complex assembly involves the
formation of a D-B or D-A-B complex, which consists of TFIID, TFIIB (TFIIA) and
the promoter DNA. The stability of the D-B and D-A-B complexes is thought to
be greater than that of TFIID and DNA alone. The full-length human cDNA for
TFIIB is expressed in E. coli and has a molecular weight of 32kDa. TFIIB alone
does not have DNA-binding activity.
Features:
• Performance-Tested: Tested by gel shift assay for the formation of the
D-B complex. Tested for in vitro transcriptional activity.
Storage Conditions: Store at –70°C.
Transcription Factor Consensus
Oligonucleotides
Product Size Conc. Cat.# Price ($)
AP1 Consensus Oligonucleotide 175 pmol 1.75 pmol/µl E3201 172.00
35 pmol 1.75 pmol/µl E3202 54.00
AP2 Consensus Oligonucleotide 175 pmol 1.75 pmol/µl E3211 172.00
35 pmol 1.75 pmol/µl E3212 54.00
CREB Consensus
175 pmol 1.75 pmol/µl E3281 172.00
Oligonucleotide
35 pmol 1.75 pmol/µl E3282 54.00
NF-κB Consensus
175 pmol 1.75 pmol/µl E3291 172.00
Oligonucleotide
35 pmol 1.75 pmol/µl E3292 54.00
OCT1 Consensus Oligonucleotide 175 pmol 1.75 pmol/µl E3241 172.00
35 pmol 1.75 pmol/µl E3242 54.00
SP1 Consensus Oligonucleotide 175 pmol 1.75 pmol/µl E3231 172.00
35 pmol 1.75 pmol/µl E3232 54.00
TFIID Consensus Oligonucleotide 175 pmol 1.75 pmol/µl E3221 172.00
35 pmol 1.75 pmol/µl E3222 54.00
For Research Use Only. Not for Use in Diagnostic Procedures.
Description: The electrophoretic mobility shift assay (EMSA, gel shift, gel
retardation) is a relatively simple and sensitive method to investigate
protein:DNA interactions. These oligonucleotides contain consensus DNAbinding
sites for individual sequence-specific transcription factors. The doublestranded
oligonucleotides are designed with 5´ OH blunt ends, making them
easily labeled to high specific activity with T4 polynucleotide kinase.
Storage Conditions: Store at –20°C.
Protocol
Gel Shift Assay System Technical Bulletin
Part#
TB110
Characteristics of the Consensus Oligonucleotides and Binding
Proteins.
AP1 (c-jun) 5ʹ-CGC TTG ATG AGT CAG CCG GAA-3ʹ
3ʹ-GCG AAC TAC TCA GTC GGC CTT-5ʹ
Forms DNA binding dimers with other members of the AP1 family and with Fos through leucine
zipper formation.
AP2
5ʹ-GAT CGA ACT GAC CGC CCG CGG CCC GT-3ʹ
3ʹ-CTA GCT TGA CTG GCG GGC GCC GGG CA-5ʹ
May act independently as both a TPA- and cAMP-inducible element and can be
specifi cally inhibited by large T antigen.
CREB
5ʹ-AGA GAT TGC CTG ACG TCA GAG AGC TAG-3ʹ
3ʹ-TCT CTA ACG GAC TGC AGT CTC TCG ATC-5ʹ
Confers responsiveness to cAMP; it contains a leucine zipper motif for dimerization, and the
associated basic domain is homologous to c-Jun DNA binding domains.
NF-κB
5ʹ-AGT TGA GGG GAC TTT CCC AGG C-3ʹ
3ʹ-TCA ACT CCC CTG AAA GGG TCC G-5ʹ
Binds to κ light chain enhancer in B cells and is present in a covert cytoplasmic form in
non-B cells.
OCT1
5ʹ-TGT CGA ATG CAA ATC ACT AGA A-3ʹ
3ʹ-ACA GCT TAC GTT TAG TGA TCT T-5ʹ
A member of the OCT family, which is apparently ubiquitous in mammalian cells, the bipartite
POU domain includes the POU-box and the homeo domain.
SP1
5ʹ-ATT CGA TCG GGG CGG GGC GAG C-3ʹ
3ʹ-TAA GCT AGC CCC GCC CCG CTC G-5ʹ
O-glycosylated transcription factor with sequence specifi city conferred through three zinc fi ngers
in the DNA binding domain.
TFIID
5ʹ-GCA GAG CAT ATA AGG TGA GGT AGG A-3ʹ
3ʹ-CGT CTC GTA TAT TCC ACT CCA TCC T-5ʹ
A general transcription factor that exhibits specifi c DNA binding to the TATA box.
This factor is associated with RNA polymerase I, II and III activities.
9491LA
Table of
Contents
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