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<strong>Life</strong> <strong>Science</strong> Catalog 2012 Worldwide Contact List Cell Signaling pTargeT Mammalian Expression Vector System Product Size Cat.# Price ($) pTargeT Mammalian Expression Vector System 20 reactions A1410 569.00 For Research Use Only. Not for Use in Diagnostic Procedures. Description: The pTargeT Mammalian Expression Vector System is a convenient system for cloning PCR products and for expression of cloned PCR products in mammalian cells. The vector is prepared by digestion with EcoRV followed by addition of a 3´ terminal thymidine to each end. These single 3´-T overhangs at the insertion site greatly improve the efficiency of ligation of a PCR product into the plasmid in two ways. First, the overhangs prevent recircularization of the vector; second, they provide a compatible overhang for PCR products generated by certain thermostable polymerases. These polymerases often add a single deoxyadenosine, in a template-independent fashion, to the 3´-ends of amplified fragments. The pTargeT Vector also contains a modified version of the coding sequence of the α-peptide of β-galactosidase, which allows recombinants to be selected using blue/white screening.The pTargeT Vector carries the human cytomegalovirus (CMV) immediate-early enhancer/promoter region to promote constitutive expression of cloned DNA inserts in mammalian cells. This vector also contains the neomycin phosphotransferase gene, a selectable marker for mammalian cells. The pTargeT Vector can be used for transient expression or for stable expression by selecting transfected cells with the antibiotic G-418. Features: • Simple PCR Cloning: “T” overhangs permit direct ligation of PCR products generated by thermostable enzymes such as Taq DNA polymerase. • Strong, Constitutive Expression: The CMV enhancer/promoter region allows strong, constitutive expression in many cell types. In transgenic mice, expression of the chloramphenicol acetyltransferase (CAT) gene under the regulation of the CMV enhancer/promoter was observed in 24 of the 28 tissues examined. The vector is maintained as an episome in cells expressing the SV40 large T antigen, leading to even higher levels of expression. • Blue/White Screening: Allows the easy identification of recombinant clones. A single digest removes the insert DNA. • Stable Transfectants: Select for stable transfectants using the neomycin phosphotransferase gene. Storage Conditions: Store competent cells at –70°C; store all other components at –20°C or –70°C. Protocol pTARGET Mammalian Expression Vector System Technical Manual Part# TM044 pTargeT Sequencing Primer Product Size Cat.# Price ($) pTargeT Sequencing Primer 2 μg Q4461 134.00 For Research Use Only. Not for Use in Diagnostic Procedures. Description: The pTargeT Sequencing Primer is designed for sequencing inserts cloned into the pTargeT Mammalian Expression Vector (Cat.# A1410). The sequencing primer hybridizes to the region of the lacZ gene from nucleotides 1367–1344 on the pTargeT Vector. The primer can be used only for sequencing inserts cloned into the pTargeT Vector. The primer sequence is not a binding site for any RNA polymerases and cannot be used to generate in vitro transcripts. The sequence of the pTargeT Sequencing Primer is 5´-d(TTACGCCAAGTTAT TTAGGTGACA)-3´. The primer is supplied at a concentration of 10ng/μl (1.25pmol/μl) in sterile water. Storage Conditions: Store at –20°C. BglII 5665 Ampr ori Synthetic poly(A) Neo CMV Enhancer/Promoter pTARGET Vector (5670bp) Intron fl ori SV40 Enhancer/ EarlyPromoter SgfI 664 SV40 Late poly (A) I-PpoI 851 T T lacZ T7 EcoRI BamHI NheI XhoI MluI SmaI KpnI SalI AccI NotI EcoRI lacZ ➞ T overhangs 1250 1256 1264 1270 1276 1293 1301 1303 1304 1311 1318 1505VA07_6A Section Contents Table of Contents 220 For complete and up-to-date product information visit: www.promega.com/catalog
Cell Signaling 13 Protein Expression and Analysis 13 Eukaryotic Cell-Free Protein Expression Prokaryotic Cell-Free Protein Expression 222 228 Cell-Based Protein Expression 229 Mass Spectrometry Analysis 230 Protein Labeling and Detection 234 Protein Expression and Analysis Section Contents For complete and up-to-date product information visit: www.promega.com/catalog 221 Table of Contents
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Life Science CATALOG 2012
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Cell Signaling Table of Contents Pr
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Cell Signaling 1 Biochemicals and L
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Cell Signaling Agarose, LMP, Prepar
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Cell Signaling Blue/Orange Loading
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Cell Signaling Glycine, Molecular B
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Cell Signaling Sephacryl ® S-400 P
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Cell Signaling Tris Base, Molecular
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Cell Signaling Unmethylated Lambda
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Cell Signaling Promega Barrier Tips
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Cell Signaling Product Size Cat.# P
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Cell Signaling 2 Cell Health Assays
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Cell Signaling A. B. Luminescence/p
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Cell Signaling Luminogenic Enzyme S
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Cell Signaling Apoptosis
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Cell Signaling Caspase-Glo ® 2 Ass
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Cell Signaling Caspase-Glo ® 9 Ass
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Cell Signaling CaspACE Assay System
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Cell Signaling Anti-ACTIVE ® Caspa
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Cell Signaling BacTiter-Glo Microbi
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Cell Signaling CellTiter 96 ® AQ u
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Cell Signaling MultiTox-Fluor Multi
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Cell Signaling CytoTox-Glo Cytotoxi
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Cell Signaling CytoTox 96 ® Non-Ra
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Cell Signaling GSH-Glo Glutathione
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Cell Signaling 3 Cell Signaling 3 G
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Cell Signaling cAMP-Glo Max Assay P
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Cell Signaling Growth Factors Epide
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Cell Signaling SIRT-Glo Assays and
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Cell Signaling ADP-Glo Max Assay Pr
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Cell Signaling Kinase Enzyme System
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Cell Signaling Kinase Enzyme System
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Cell Signaling Kinase-Glo ® Lumine
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Cell Signaling SignaTECT ® Protein
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Cell Signaling Casein Kinase I Prod
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Cell Signaling Product Size Cat.# P
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Cell Signaling Protein Kinase Subst
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Cell Signaling 4 Cloning and DNA Ma
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Cell Signaling DNA Step Ladders Pro
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Cell Signaling Conventional DNA Mar
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Cell Signaling ProMega-Markers ® L
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Cell Signaling Restriction Enzymes
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Cell Signaling BamHI 37 Product Siz
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Cell Signaling EcoRI 37 Product Siz
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Cell Signaling NciI 37 Product Size
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Cell Signaling SpeI 37 Product Size
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Cell Signaling TSAP Thermosensitive
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Cell Signaling T3 RNA Polymerase Pr
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Cell Signaling Kinases T4 Polynucle
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Cell Signaling S1 Nuclease Product
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Cell Signaling Percent Inhibition o
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Cell Signaling Untagged Flexi ® Ma
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Cell Signaling pGEM ® -5Zf(+) Vect
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Cell Signaling pSP64 Poly(A) Vector
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Cell Signaling 5 DNA and RNA Purifi
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Cell Signaling x-tracta Gel Extract
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Cell Signaling Wizard ® MagneSil
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Cell Signaling ReliaPrep FFPE gDNA
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Cell Signaling MagneSil ® ONE, Fix
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Cell Signaling MagneSil ® KF, Geno
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Cell Signaling Plasmid Purification
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Cell Signaling PureYield Plasmid Ma
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Cell Signaling Wizard ® Plus Maxip
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Cell Signaling Wizard ® MagneSil
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Cell Signaling PureYield RNA Midipr
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Cell Signaling Maxwell ® 16 System
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Cell Signaling RNasin ® Plus RNase
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Cell Signaling DNA Quantitation Qua
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Cell Signaling Magnetic Stands and
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Cell Signaling 6 Drug Discovery 6 D
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Cell Signaling GPCR Assays cAMP-Glo
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Cell Signaling PDE-Glo Phosphodiest
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Cell Signaling Protease Assays Prot
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Cell Signaling Cell-Based Proteasom
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Cell Signaling Tryptase, Human, Rec
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Cell Signaling 7 Genetic Identity 7
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Cell Signaling Maxwell ® 16 Forens
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Cell Signaling Plexor ® HY System
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Cell Signaling PowerPlex ® 18D Sys
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Cell Signaling PowerPlex ® CS7 Sys
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Cell Signaling PowerPlex ® 16 and
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Cell Signaling GenePrint ® Fluores
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Cell Signaling 8 Imaging and Immuno
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Cell Signaling HaloTag ® Ligand Bu
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Cell Signaling Block & Sample 5X Bu
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Cell Signaling Anti-ACTIVE ® JNK p
Page 173 and 174: Cell Signaling Anti-ERK 1/2 pAb, Ra
Page 175 and 176: Cell Signaling Anti-Human GDNF pAb
Page 177 and 178: Cell Signaling Anti-PARP p85 Fragme
Page 179 and 180: Cell Signaling Anti-VAChT pAb Produ
Page 181 and 182: Cell Signaling VivoGlo Caspase 3/7
Page 183 and 184: Cell Signaling 9 Industrial and Env
Page 185 and 186: Cell Signaling Microbial Detection
Page 187 and 188: 10 Instruments 10 Luminometers 184
Page 189 and 190: GloMax ® 20/20 Luminometer Product
Page 191 and 192: Fluorescence Module: Application-op
Page 193 and 194: GloMax ® -Multi Jr Single-Tube Mul
Page 195 and 196: Maxwell ® 16 Instruments Maxwell
Page 197 and 198: Maxwell ® 16 Flexi Method Firmware
Page 199 and 200: Cell Signaling 11 Molecular Diagnos
Page 201 and 202: Cell Signaling Maxwell ® 16 System
Page 203 and 204: Cell Signaling Maxwell ® 16 Flexi
Page 205 and 206: Cell Signaling dNTP Mix Product Siz
Page 207 and 208: Cell Signaling 12 PCR 12 Hot-Start
Page 209 and 210: Cell Signaling TaqBead Hot Start Po
Page 211 and 212: Cell Signaling GoTaq ® PCR Core Sy
Page 213 and 214: Cell Signaling Deoxynucleotide Trip
Page 215 and 216: Cell Signaling Plexor ® qPCR and q
Page 217 and 218: Cell Signaling StemElite Differenti
Page 219 and 220: Cell Signaling Reverse Transcriptio
Page 221 and 222: Cell Signaling AMV Reverse Transcri
Page 223: Cell Signaling PCR Cloning pGEM ®
Page 227 and 228: Cell Signaling TnT ® Quick Coupled
Page 229 and 230: Cell Signaling TnT ® T7 Quick for
Page 231 and 232: Cell Signaling Canine Pancreatic Mi
Page 233 and 234: Cell Signaling pGEM ® β-Gal Contr
Page 235 and 236: Cell Signaling Immobilized Trypsin
Page 237 and 238: Cell Signaling Asp-N, Sequencing Gr
Page 239 and 240: Cell Signaling HaloTag ® Ligand Bu
Page 241 and 242: Cell Signaling Inducible T7-Driven
Page 243 and 244: Cell Signaling ProtoBlot ® II AP S
Page 245 and 246: Cell Signaling 14 Protein Purificat
Page 247 and 248: Cell Signaling HaloTag ® Protein P
Page 249 and 250: HT Cell Signaling HaloTag ® Mammal
Page 251 and 252: Cell Signaling HaloLink Protein Arr
Page 253 and 254: Cell Signaling HaloCHIP System Prod
Page 255 and 256: Cell Signaling MagneHis Protein Pur
Page 257 and 258: Cell Signaling Streptavidin Product
Page 259 and 260: Cell Signaling Protein Purification
Page 261 and 262: Cell Signaling 15 Reporter Assays a
Page 263 and 264: Cell Signaling Dual-Glo ® Lucifera
Page 265 and 266: Cell Signaling Firefly Luciferase R
Page 267 and 268: Cell Signaling Bright-Glo Luciferas
Page 269 and 270: Cell Signaling EnduRen Live Cell Su
Page 271 and 272: Cell Signaling Beta-Glo ® Assay Sy
Page 273 and 274: Cell Signaling Promoter-Driven Cont
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Cell Signaling Nuclear Receptor Pat
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Cell Signaling pRL Renilla Lucifera
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Cell Signaling GloResponse Lucifera
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Cell Signaling pSP-luc+NF Fusion Ve
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Cell Signaling VivoGlo Caspase 3/7
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Cell Signaling Transfection Reagent
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Cell Signaling 16 RNA Analysis 16 I
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Cell Signaling Riboprobe ® System
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Cell Signaling HeLaScribe ® Nuclea
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Cell Signaling RNA Interference Gen
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Cell Signaling 17 Stem Cell Researc
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Cell Signaling Cell ID System Produ
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Cell Signaling StemElite Human Panc
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Cell Signaling 18 Vectors 18 Expres
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Cell Signaling HaloTag ® Fusion (N
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Cell Signaling pCI Mammalian Expres
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Cell Signaling Reporter Vectors pGL
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Cell Signaling pGEM ® -luc DNA Pro
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Cell Signaling pGEM ® and pSP Subc
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Cell Signaling pUC/M13 Sequencing P
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Index: A-Z 19 Index 19 Index: A-Z 3
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Index: A-Z BssHII..................
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Index: A-Z In vitro Transcription S
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Index: A-Z QuantiFluor dsDNA System
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Index by Catalog Number Cat# Produc
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personal detection GloMax Integrat
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Life Science - Promega

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