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<strong>Life</strong> <strong>Science</strong> Catalog 2012 Worldwide Contact List Cell Signaling HaloLink Protein Array Systems Product Size Cat.# Price ($) HaloLink Array (TnT ® T7 Quick) Two Slide System 2 arrays G6140 649.00 HaloLink Array (TnT ® SP6 Wheat Germ) Two Slide System 2 arrays G6180 649.00 HaloLink Array Six Slide System 6 slides G6190 Pls. Enq. HaloTag ® Standard Protein 30 μg G4491 89.00 For Research Use Only. Not for Use in Diagnostic Procedures. For additional information see page 247. HaloLink Magnetic Beads Product Size Cat.# Price ($) HaloLink Magnetic Beads 40 reactions G9311 169.00 For Research Use Only. Not for Use in Diagnostic Procedures. For additional information see page 248. HaloCHIP System Product Size Cat.# Price ($) HaloCHIP System 20 reactions G9410 829.00 For Research Use Only. Not for Use in Diagnostic Procedures. For additional information see page 249. HaloLink Resin Product Size Cat.# Price ($) HaloLink Resin 1.25 ml G1912 509.00 For Research Use Only. Not for Use in Diagnostic Procedures. For additional information see page 246. 2.5 ml G1913 201.00 10 ml G1914 464.00 25 ml G1915 850.00 HaloTag ® Mammalian Pull-Down Systems Product Size Cat.# Price ($) HaloTag ® Mammalian Pull-Down and Labeling System 24 reactions G6500 521.00 HaloTag ® Mammalian Pull-Down System 24 reactions G6504 284.00 HaloTag ® Control Vector 20 μg G6591 231.00 Available Separately Protease Inhibitor Cocktail, 50X 1 ml G6521 87.00 Mammalian Lysis Buffer 40 ml G9381 58.00 For Research Use Only. Not for Use in Diagnostic Procedures. For additional information see page 245. MagneGST Pull-Down System Product Size Cat.# Price ($) MagneGST Pull-Down System 80 reactions V8870 913.00 For Research Use Only. Not for Use in Diagnostic Procedures. For additional information see page 250. Gel Shift Assay Systems Product Size Cat.# Price ($) Gel Shift Assay Core System 100 reactions E3050 592.00 Gel Shift Assay System 100 reactions E3300 767.00 Available Separately HeLaScribe ® Nuclear Extract, Gel Shift Assay Grade 3 × 40 μl E3521 317.00 Gel Shift Binding 5X Buffer 5 × 200 μl E3581 487.00 For Research Use Only. Not for Use in Diagnostic Procedures. Description: The gel shift or electrophoretic mobility shift assay provides a simple and rapid method for detecting DNA-binding proteins. This method is widely used to study sequence-specific DNA-binding proteins such as transcription factors. The assay is based on the observation that complexes of protein and DNA migrate through a nondenaturing polyacrylamide gel more slowly than free DNA fragments or double-stranded oligonucleotides. The gel shift assay is performed by incubating a purified protein or a complex mixture of proteins (such as nuclear or cell extract preparations) with a 32 P end-labeled DNA fragment containing the putative protein binding site. The reaction products are then analyzed on a nondenaturing polyacrylamide gel. The specificity of the DNA-binding protein for the putative binding site is established by competition experiments using unlabeled DNA fragments or oligonucleotides containing a binding site for the protein of interest or other unrelated DNA sequences. The Core System (Cat.# E3050) includes HeLa Nuclear Extract and SP1 and AP2 Consensus Oligos that can be used as positive controls and serve as a reliable system for obtaining experience with gel shift assays. In addition, the Core System contains T4 Polynucleotide Kinase and Kinase 10X Buffer for labeling oligonucleotides as well as Gel Shift Binding 5X Buffer. Cat.# E3300 contains all of the above plus consensus oligos for AP1, OCT1, CREB, NF-κB, and TFIID. Features: • Positive Controls: The Gel Shift Assay Core System includes a HeLa Nuclear Extract and consensus oligonucleotides for AP2 and SP1. • Versatile: Oligonucleotides can be 5´ end-labeled and used as proteinspecific probes or used as unlabeled oligonucleotides in competition assays. Storage Conditions: Store HeLa Nuclear Extract at –70°C. Store other components at –20°C. Protocol Gel Shift Assay System Technical Bulletin Part# TB110 Section Contents Table of Contents 254 For complete and up-to-date product information visit: www.promega.com/catalog
Cell Signaling Protein Purification Accessories Magnetic Stands and Spacers Product Size Cat.# Price ($) MagnaBot ® 384 Magnetic Separation Device 1 each V8241 Pls. Enq. 384-Well Plate, Flat 10 /pk V5291 Pls. Enq. 384-Well Plate, Conical 10 /pk V5311 Pls. Enq. MagnaBot ® 96 Magnetic Separation Device (Cat.# V8151) with a 96- well Collection Plate and robotic gripper arm. Product Size Cat.# Price ($) MagnaBot ® 96 Magnetic Separation Device 1 each V8151 813.00 MagnaBot ® II Magnetic Separation Device 1 each V8351 Pls. Enq. MagnaBot ® Flat Top Magnetic Separation Device 1 each V6041 Pls. Enq. Plate Clamp 96 1 each V8251 Pls. Enq. Plate Stand 1 each V8261 Pls. Enq. MagnaBot ® Adapter T1 1 each V8481 Pls. Enq. 2889TA03_0A Plate Stand (Cat.# V8261). Plate Clamp 96 (Cat.# V8251) with a 96-well PCR plate. 3373TA05_1A 3375TA05_1A 14 Protein Purification and Interactions Deep Well MagnaBot ® 96 Magnetic Separation Device 1 each V3031 Pls. Enq. Heat Transfer Block 1 each Z3271 96.00 Heat Block Insert 1 each Z3651 170.00 3993TA02_3A 3995TA02_3A MagnaBot ® 384 Magnetic Separation Device (Cat.# V8241). MagnaBot ® 96 Magnetic Separation Device (Cat.# V8151). 3417TA05_1A Section Contents MagnaBot ® II Magnetic Separation Device (Cat.# V8351). For complete and up-to-date product information visit: www.promega.com/catalog 255 Table of Contents
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Life Science CATALOG 2012
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Cell Signaling Table of Contents Pr
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Cell Signaling 1 Biochemicals and L
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Cell Signaling Agarose, LMP, Prepar
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Cell Signaling Blue/Orange Loading
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Cell Signaling Glycine, Molecular B
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Cell Signaling Sephacryl ® S-400 P
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Cell Signaling Tris Base, Molecular
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Cell Signaling Unmethylated Lambda
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Cell Signaling Promega Barrier Tips
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Cell Signaling Product Size Cat.# P
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Cell Signaling 2 Cell Health Assays
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Cell Signaling A. B. Luminescence/p
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Cell Signaling Luminogenic Enzyme S
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Cell Signaling Apoptosis
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Cell Signaling Caspase-Glo ® 2 Ass
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Cell Signaling Caspase-Glo ® 9 Ass
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Cell Signaling CaspACE Assay System
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Cell Signaling Anti-ACTIVE ® Caspa
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Cell Signaling BacTiter-Glo Microbi
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Cell Signaling CellTiter 96 ® AQ u
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Cell Signaling MultiTox-Fluor Multi
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Cell Signaling CytoTox-Glo Cytotoxi
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Cell Signaling CytoTox 96 ® Non-Ra
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Cell Signaling GSH-Glo Glutathione
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Cell Signaling 3 Cell Signaling 3 G
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Cell Signaling cAMP-Glo Max Assay P
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Cell Signaling Growth Factors Epide
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Cell Signaling SIRT-Glo Assays and
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Cell Signaling ADP-Glo Max Assay Pr
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Cell Signaling Kinase Enzyme System
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Cell Signaling Kinase Enzyme System
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Cell Signaling Kinase-Glo ® Lumine
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Cell Signaling SignaTECT ® Protein
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Cell Signaling Casein Kinase I Prod
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Cell Signaling Product Size Cat.# P
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Cell Signaling Protein Kinase Subst
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Cell Signaling 4 Cloning and DNA Ma
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Cell Signaling DNA Step Ladders Pro
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Cell Signaling Conventional DNA Mar
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Cell Signaling ProMega-Markers ® L
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Cell Signaling Restriction Enzymes
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Cell Signaling BamHI 37 Product Siz
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Cell Signaling EcoRI 37 Product Siz
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Cell Signaling NciI 37 Product Size
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Cell Signaling SpeI 37 Product Size
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Cell Signaling TSAP Thermosensitive
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Cell Signaling T3 RNA Polymerase Pr
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Cell Signaling Kinases T4 Polynucle
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Cell Signaling S1 Nuclease Product
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Cell Signaling Percent Inhibition o
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Cell Signaling Untagged Flexi ® Ma
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Cell Signaling pGEM ® -5Zf(+) Vect
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Cell Signaling pSP64 Poly(A) Vector
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Cell Signaling 5 DNA and RNA Purifi
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Cell Signaling x-tracta Gel Extract
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Cell Signaling Wizard ® MagneSil
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Cell Signaling ReliaPrep FFPE gDNA
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Cell Signaling MagneSil ® ONE, Fix
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Cell Signaling MagneSil ® KF, Geno
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Cell Signaling Plasmid Purification
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Cell Signaling PureYield Plasmid Ma
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Cell Signaling Wizard ® Plus Maxip
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Cell Signaling Wizard ® MagneSil
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Cell Signaling PureYield RNA Midipr
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Cell Signaling Maxwell ® 16 System
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Cell Signaling RNasin ® Plus RNase
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Cell Signaling DNA Quantitation Qua
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Cell Signaling Magnetic Stands and
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Cell Signaling 6 Drug Discovery 6 D
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Cell Signaling GPCR Assays cAMP-Glo
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Cell Signaling PDE-Glo Phosphodiest
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Cell Signaling Protease Assays Prot
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Cell Signaling Cell-Based Proteasom
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Cell Signaling Tryptase, Human, Rec
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Cell Signaling 7 Genetic Identity 7
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Cell Signaling Maxwell ® 16 Forens
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Cell Signaling Plexor ® HY System
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Cell Signaling PowerPlex ® 18D Sys
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Cell Signaling PowerPlex ® CS7 Sys
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Cell Signaling PowerPlex ® 16 and
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Cell Signaling GenePrint ® Fluores
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Cell Signaling 8 Imaging and Immuno
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Cell Signaling HaloTag ® Ligand Bu
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Cell Signaling Block & Sample 5X Bu
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Cell Signaling Anti-ACTIVE ® JNK p
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Cell Signaling Anti-ERK 1/2 pAb, Ra
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Cell Signaling Anti-Human GDNF pAb
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Cell Signaling Anti-PARP p85 Fragme
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Cell Signaling Anti-VAChT pAb Produ
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Cell Signaling VivoGlo Caspase 3/7
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Cell Signaling 9 Industrial and Env
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Cell Signaling Microbial Detection
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10 Instruments 10 Luminometers 184
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GloMax ® 20/20 Luminometer Product
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Fluorescence Module: Application-op
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GloMax ® -Multi Jr Single-Tube Mul
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Maxwell ® 16 Instruments Maxwell
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Maxwell ® 16 Flexi Method Firmware
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Cell Signaling 11 Molecular Diagnos
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Cell Signaling Maxwell ® 16 System
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Cell Signaling Maxwell ® 16 Flexi
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Cell Signaling dNTP Mix Product Siz
Page 207 and 208: Cell Signaling 12 PCR 12 Hot-Start
Page 209 and 210: Cell Signaling TaqBead Hot Start Po
Page 211 and 212: Cell Signaling GoTaq ® PCR Core Sy
Page 213 and 214: Cell Signaling Deoxynucleotide Trip
Page 215 and 216: Cell Signaling Plexor ® qPCR and q
Page 217 and 218: Cell Signaling StemElite Differenti
Page 219 and 220: Cell Signaling Reverse Transcriptio
Page 221 and 222: Cell Signaling AMV Reverse Transcri
Page 223 and 224: Cell Signaling PCR Cloning pGEM ®
Page 225 and 226: Cell Signaling 13 Protein Expressio
Page 227 and 228: Cell Signaling TnT ® Quick Coupled
Page 229 and 230: Cell Signaling TnT ® T7 Quick for
Page 231 and 232: Cell Signaling Canine Pancreatic Mi
Page 233 and 234: Cell Signaling pGEM ® β-Gal Contr
Page 235 and 236: Cell Signaling Immobilized Trypsin
Page 237 and 238: Cell Signaling Asp-N, Sequencing Gr
Page 239 and 240: Cell Signaling HaloTag ® Ligand Bu
Page 241 and 242: Cell Signaling Inducible T7-Driven
Page 243 and 244: Cell Signaling ProtoBlot ® II AP S
Page 245 and 246: Cell Signaling 14 Protein Purificat
Page 247 and 248: Cell Signaling HaloTag ® Protein P
Page 249 and 250: HT Cell Signaling HaloTag ® Mammal
Page 251 and 252: Cell Signaling HaloLink Protein Arr
Page 253 and 254: Cell Signaling HaloCHIP System Prod
Page 255 and 256: Cell Signaling MagneHis Protein Pur
Page 257: Cell Signaling Streptavidin Product
Page 261 and 262: Cell Signaling 15 Reporter Assays a
Page 263 and 264: Cell Signaling Dual-Glo ® Lucifera
Page 265 and 266: Cell Signaling Firefly Luciferase R
Page 267 and 268: Cell Signaling Bright-Glo Luciferas
Page 269 and 270: Cell Signaling EnduRen Live Cell Su
Page 271 and 272: Cell Signaling Beta-Glo ® Assay Sy
Page 273 and 274: Cell Signaling Promoter-Driven Cont
Page 275 and 276: Cell Signaling Nuclear Receptor Pat
Page 277 and 278: Cell Signaling pRL Renilla Lucifera
Page 279 and 280: Cell Signaling GloResponse Lucifera
Page 281 and 282: Cell Signaling pSP-luc+NF Fusion Ve
Page 283 and 284: Cell Signaling VivoGlo Caspase 3/7
Page 285 and 286: Cell Signaling Transfection Reagent
Page 287 and 288: Cell Signaling 16 RNA Analysis 16 I
Page 289 and 290: Cell Signaling Riboprobe ® System
Page 291 and 292: Cell Signaling HeLaScribe ® Nuclea
Page 293 and 294: Cell Signaling RNA Interference Gen
Page 295 and 296: Cell Signaling 17 Stem Cell Researc
Page 297 and 298: Cell Signaling Cell ID System Produ
Page 299 and 300: Cell Signaling StemElite Human Panc
Page 301 and 302: Cell Signaling 18 Vectors 18 Expres
Page 303 and 304: Cell Signaling HaloTag ® Fusion (N
Page 305 and 306: Cell Signaling pCI Mammalian Expres
Page 307 and 308: Cell Signaling Reporter Vectors pGL
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Cell Signaling pGEM ® -luc DNA Pro
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Cell Signaling pGEM ® and pSP Subc
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Cell Signaling pUC/M13 Sequencing P
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Index: A-Z 19 Index 19 Index: A-Z 3
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Index: A-Z BssHII..................
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Index: A-Z In vitro Transcription S
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Index: A-Z QuantiFluor dsDNA System
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Index by Catalog Number Cat# Produc
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personal detection GloMax Integrat
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Life Science - Promega

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