Link to our lab as a pdf - College of Science - Marshall University

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The People Involved The Mondelos Louise Ann Mondelo is the 42 year old mother and wife of the Mondelo family. She is also one of the owners of Tumbling Water Land Development Company. Friends say that Louise was in an unhappy marriage and had recently filed for divorce. Lyle Christopher Mondelo is the 44 year old husband and father of the Mondelo family. He is a part owner of Tumbling Water Land Development Company and, along with his wife, a partner in L&L Mondelo. Wally Christopher Mondelo, is the oldest child of Lyle & Louise, at 14 years old. During the time that Wally was conceived, Lyle suspected Louise of cheating on him. Jan Marie Mondelo is 11 years old. Other People John Wayne Gretzky is 43 years old. He is a friend and business partner of the Mondelo’s in the Tumbling Water Land Development Company. Rumors have it that John Wayne and Louise had a brief affair when Lyle and Louise first moved to Highland Park. He is known around town to be a greedy businessman, and has been suspected of shady deals in the past. An unknown woman of similar height and build has been identified as Louise Mondelo. Although her identity is uncertain, this other woman was found either driving the Mondelo family car with two children preliminarily identified as Wally and Jan, or in a remote fishing cabin with a man who has been preliminarily identified as Louise’s husband Lyle Mondelo. The Mystery of Lyle and Louise 23
Pre-Lab Questions DNA Background 1. What is the key difference between PCR and RFLP? 2. Why does this difference make PCR better suited for forensics? 3. What causes DNA to move during electrophoresis? 4. How is the DNA sorted after electrophoresis? 5. What is a Ladder? Procedure 6. How much DNA goes into each lane? 7. What always goes in the first lane of the gel? 8. Why is it important to write down the loading order of the samples in the wells? 9. Which direction does DNA travel? Should the wells be on the end near the black or red electrode? The Mystery of Lyle and Louise 25
Page 1 and 2: An Identity Crisis A Lab on DNA Typ
Page 3 and 4: Table of Contents Introduction to I
Page 5 and 6: Introduction to Identity Crisis WEL
Page 7 and 8: These notes are provided to assist
Page 9 and 10: Glossary Agarose: A molecule purifi
Page 11 and 12: PCR Technology PCR is the cornersto
Page 13 and 14: Forensic DNA Technology stabilizes
Page 15 and 16: 18 The Mystery of Lyle and Louise
Page 17 and 18: The Investigation this time, invest
Page 19: The Evidence Evidence was collected
Page 23 and 24: Lab Procedure 6. 7. 8. 9. to go dow
Page 25 and 26: Post-Lab Questions Short Answer 1.
Page 27: Mock Trial documents prepared by th

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