Link to our lab as a pdf - College of Science - Marshall University

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4 The Mystery of Lyle and Louise
Introduction to Identity Crisis WELCOME to Identity Crisis, a DNA Typing lab in the Mystery of Lyle and Louise. A brutal murder case is unfolding in a small Appalachian town. Already the case spans two crime scenes and five people are dead. However, two women, one at each crime scene, have been identified as Louise Mondelo. Your students, as the forensic investigators responsible for the DNA evidence, must positively identify Louise Mondelo and also analyze the DNA evidence for clues that might help crack the case. In this lab, students will learn about DNA Typing and related technologies. These include the polymerase chain reaction (PCR), a method for amplifying small samples of DNA, and electrophoresis, a method for separating bands within the PCR product. An introduction to short tandem repeat DNA analysis is also covered in this booklet. In addition to reading information provided about the case, students will test DNA samples from people and objects, compare them to each other, and make assessments of the probability that samples came from the same source. Once the lab results have been analyzed, students may host a mock trial to hold a chosen suspect accountable for their actions. Teacher’s notes can be found at the beginning of the manual and copies may be freely made of all materials for your students. The Mystery of Lyle and Louise 5
Page 1 and 2: An Identity Crisis A Lab on DNA Typ
Page 3: Table of Contents Introduction to I
Page 7 and 8: These notes are provided to assist
Page 9 and 10: Glossary Agarose: A molecule purifi
Page 11 and 12: PCR Technology PCR is the cornersto
Page 13 and 14: Forensic DNA Technology stabilizes
Page 15 and 16: 18 The Mystery of Lyle and Louise
Page 17 and 18: The Investigation this time, invest
Page 19 and 20: The Evidence Evidence was collected
Page 21 and 22: Pre-Lab Questions DNA Background 1.
Page 23 and 24: Lab Procedure 6. 7. 8. 9. to go dow
Page 25 and 26: Post-Lab Questions Short Answer 1.
Page 27: Mock Trial documents prepared by th

Link to our lab as a pdf - College of Science - Marshall University

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