Link to our lab as a pdf - College of Science - Marshall University

Lab Procedure
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to go down, but you should notice a distinct
change in resistance.
Place the tip of the pipette into the liquid in the
tube, but do not touch the bottom. Release the
plunger to obtain the contents of the tube.
Carefully hover the tip into the appropriate
well in the gel, being careful not to touch the
gel itself.
Press the plunger down slowly to dispense the
sample into the well. Do not touch the gel itself.
Remove the pipette from the well before
releasing the plunger.
Discard the used tip.
10. Record the location of the sample on your Data
Collection sheet.
11. Moving from left to right, repeat these steps
for each sample, using a different well and tip
each time.
Running the Gel (45 min)
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Place the lid down on the top of the gel box.
Check to see that the black electrode matches
up with the black lead and red with red. Plug
the leads into the power supply, also matching
the colors.
Before turning on the power supply, make sure
it reads “Volts” and the setting is “Low.”
Once all lab stations have been checked, turn
on the power supply and twist the knob until
the display reads the voltage that your instructor
suggests (usually between 60 and 100 volts)
You should see the orange dye move out of the
wells within a few minutes. If it has not moved,
check that your power supply is plugged in, is
turned on, and is set to the correct voltage. If it
is, and there is still no movement after several
minutes, alert your instructor.
As the gel runs, you will be able to see the DNA
migrate through the gel. It will begin as a faint
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blue streak near the wells.
After the gel has run for a time, the blue bands
should have separated, and grown darker. The
blue color will intensify as the DNA stains
while the gel runs. The DNA will never become
strongly contrasted against the gel, but should
be clearly visible.
Run the gel until the orange color in the loading
dye has migrated about 75% of the way
through the gel.
Record the positions of the DNA on the gel on
your Data Collection sheet promptly.
The Mystery of Lyle and Louise