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Dimensional Measurement using Vision Systems - NPL Publications ...

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<strong>Measurement</strong> Good Practice Guide No. 39<br />

Figure 6: Image formation in the microscope<br />

2.4.1 Achromatic objective<br />

The achromatic objective is the type most commonly used in microscopy and is capable of<br />

giving good image quality at relatively low cost. It is designed so that light of two<br />

specified wavelengths, one in the red and the other in the blue region of the spectrum,<br />

comes to a common focus, there being some residual chromatic aberration (usually known<br />

as secondary spectrum). Image performance of an achromat is likely to be best in green<br />

light and appropriate filtration of the illumination is therefore desirable. The simplest<br />

types of achromatic objective produce an image with some curvature of field.<br />

2.4.2 Apochromatic objective<br />

In this type of objective, which is of more complex design and uses a wider range of<br />

optical materials than the achromat, light of three different wavelengths is brought to a<br />

common focus. The colour correction of the apochromat is significantly superior to that of<br />

the achromat and indeed, over the centre of its field the overall standard of performance is<br />

extremely high. The cost of an apochromat is usually several times that of an achromat of<br />

comparable numerical aperture.<br />

As a compromise between these two objective types, objectives known as 'fluorites' or<br />

'semi-apochromats' are available from most manufacturers. These contain fewer optical<br />

components than the true apochromat and are correspondingly cheaper; their<br />

performance is somewhat superior to that of the achromat.<br />

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