Image processing using ZEN software

Different ways of displaying the data – whilst these controls
require the image to be ‘processed’ the processing cannot
be exported unless you choose to file/export/contents of
image window

A 3D reconstruction of the data. If the image has not been
rotated this will give the same result as a maximum
intensity projection. Otherwise it can be rotated to ‘see’
around the specimen in 3D

Image processing is filed under the processing
tab (everything that is done ‘post acquisition’)
Tab to produce a MIP
from the selected data
Ensure the dataset you are
interested is the one selected
using the ‘select’ tab

To produce a subset of the dataset (this could be time or z) click the ‘copy’ option
and then select ‘subset’. Again, ensure the dataset you are interested is the one
selected using the ‘select’ function

Expand the ‘subset’ menu using the arrow and ensure you are taking a subset of z by
expanding the z menu. Press for the Gallery view of your dataset in the main window

Press ‘mouse click’ for the start of your new, clipped dataset and then click on the z-position you would
like to start with (you will still be in Gallery mode). Repeat with the End Z-position. The image window
will display the data in 2D mode (i.e. just one z-plane at a time) once you press ‘Apply’. This is now a new
image

Display your new image dataset as you
wish using the same controls as before

Beneath any image there will be an option to ‘Display’. This shows the options for
altering brightness and contract etc

In 3D mode, the ‘Appearance’ tab gives options for how to display your 3D data. If
you click on ‘Transparent’ this brings up all the tools you should need for altering
the appearance of your data