Ch 3: Observing Microorganisms Through a Microscope

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Fluorescence Microscopy

• Uses UV light.

• Fluorescent substances

absorb UV light and emit

visible light.

• Cells may be stained with

fluorescent chemicals

(fluorochromes).

• Immunofluorescence

Fig 3.6; T. pallidum

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Fluorescence Microscopy • Uses UV light. • Fluorescent substances absorb UV light and emit visible light. • Cells may be stained with fluorescent chemicals (fluorochromes). • Immunofluorescence Fig 3.6; T. pallidum

Figure 3.6a Fig 3.6b Principle of Immunofluorescence

Page 1 and 2: Ch 3: Observing Microorganisms Thro
Page 3 and 4: Objectives Review the metric units
Page 5 and 6: Sizes Among Microorganisms • Prot
Page 7 and 8: Refractive Index Fig 3.3 • Measur
Page 9: Spirochetes (Treponema pallidum) vi
Page 13 and 14: SEM or TEM? Bacterial division Leaf
Page 15 and 16: Rod-shaped Mycobacterium avium
Page 17 and 18: Simple Stains • Use a single basi
Page 19 and 20: Gram Stain crystal violet safranin
Page 21: Special Stains See Fig 3.14 • End

Ch 3: Observing Microorganisms Through a Microscope

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