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Ch 3: Observing Microorganisms Through a Microscope

Ch 3: Observing Microorganisms Through a Microscope

Ch 3: Observing Microorganisms Through a Microscope

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Preparation of Specimens for Light<br />

Microscopy<br />

• Staining Techniques Provide Contrast<br />

• Smear air-dry heat-fix<br />

• Basic dyes: cationic chromophore<br />

• Acidic dyes: anionic chromophore <br />

negative staining (good for capsules)<br />

• Three types of staining techniques:<br />

Simple, differential, and special

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