Ch 3: Observing Microorganisms Through a Microscope
Ch 3: Observing Microorganisms Through a Microscope
Ch 3: Observing Microorganisms Through a Microscope
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Preparation of Specimens for Light<br />
Microscopy<br />
• Staining Techniques Provide Contrast<br />
• Smear air-dry heat-fix<br />
• Basic dyes: cationic chromophore<br />
• Acidic dyes: anionic chromophore <br />
negative staining (good for capsules)<br />
• Three types of staining techniques:<br />
Simple, differential, and special