Ch 3: Observing Microorganisms Through a Microscope

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Principles of the Compound Light <strong>Microscope</strong> Magnification: Ocular and objective lenses of compound microscope (total mag.?) Resolution: Ability of lens to . . . Maximum resolving power ___ m Contrast: Stains change refractive index contrast between bacteria and surrounding medium Fig 3.1
Refractive Index Fig 3.3 • Measures light-bending ability of a medium • Light may bend in air so much that it misses the small high-magnification lens. • Immersion oil is used to keep light from bending.
Page 1 and 2: Ch 3: Observing Microorganisms Thro
Page 3 and 4: Objectives Review the metric units
Page 5: Sizes Among Microorganisms • Prot
Page 9 and 10: Spirochetes (Treponema pallidum) vi
Page 11 and 12: Figure 3.6a Fig 3.6b Principle of I
Page 13 and 14: SEM or TEM? Bacterial division Leaf
Page 15 and 16: Rod-shaped Mycobacterium avium
Page 17 and 18: Simple Stains • Use a single basi
Page 19 and 20: Gram Stain crystal violet safranin
Page 21: Special Stains See Fig 3.14 • End

Ch 3: Observing Microorganisms Through a Microscope

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