Ch 3: Observing Microorganisms Through a Microscope

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Electron Microscopy: Detailed Images of

Cell Parts

Uses electrons, electromagnetic lenses, and

fluorescent screens

Electron wavelength ~ 100,000 x smaller than

visible light wavelength

Specimens may be stained with heavy metal

salts

Two types of EMs:?

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Electron Microscopy: Detailed Images of Cell Parts Uses electrons, electromagnetic lenses, and fluorescent screens Electron wavelength ~ 100,000 x smaller than visible light wavelength Specimens may be stained with heavy metal salts Two types of EMs:?

SEM or TEM? Bacterial division Leaf surface

Page 1 and 2: Ch 3: Observing Microorganisms Thro
Page 3 and 4: Objectives Review the metric units
Page 5 and 6: Sizes Among Microorganisms • Prot
Page 7 and 8: Refractive Index Fig 3.3 • Measur
Page 9 and 10: Spirochetes (Treponema pallidum) vi
Page 11: Figure 3.6a Fig 3.6b Principle of I
Page 15 and 16: Rod-shaped Mycobacterium avium
Page 17 and 18: Simple Stains • Use a single basi
Page 19 and 20: Gram Stain crystal violet safranin
Page 21: Special Stains See Fig 3.14 • End

Ch 3: Observing Microorganisms Through a Microscope

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