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Ch 3: Observing Microorganisms Through a Microscope

Ch 3: Observing Microorganisms Through a Microscope

Ch 3: Observing Microorganisms Through a Microscope

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Microscopy: The Instruments<br />

Brightfield Microscopy<br />

• Simplest of all the<br />

optical microscopy<br />

illumination. techniques<br />

• Dark objects are visible<br />

against a bright<br />

background.<br />

Darkfield Microscopy<br />

• Light objects visible<br />

against dark background.<br />

• used to enhance the<br />

contrast in unstained<br />

samples.<br />

• Instrument of choice for<br />

spirochetes

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