Annals of Diagnostic Paediatric Pathology
Annals of Diagnostic Paediatric Pathology
Annals of Diagnostic Paediatric Pathology
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97<br />
3<br />
2<br />
1<br />
0<br />
x10 6 cells<br />
1 hour 4 hours<br />
Fig. 2 Digestion efficiency using 0.1% collagenase I solution was assessed<br />
using two incubation times (1 and 4 hours)<br />
4<br />
3<br />
2<br />
1<br />
0<br />
x10 6 cells<br />
A B C D E F<br />
Fig. 3 Cells obtained from 0.1% collagenase I digestion after 1 hour (a,b,c)<br />
and 4 hours (d,e,f) were checked for their growth potential in 3 different<br />
media as: - DMEM with 10% FBS (a,d); - 3:1 DMEM and F-12 mixture<br />
enriched with microsupplements and 5% FBS (b,e); - 1:1 DMEM and F-12<br />
mixture enriched with microsupplements and 2.5% FBS (c,f). Cells were<br />
counted after 4 days <strong>of</strong> culturing (A vs D, p
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