Manual: AdEasy Adenoviral Vector System - UCLA Human Genetics
Manual: AdEasy Adenoviral Vector System - UCLA Human Genetics
Manual: AdEasy Adenoviral Vector System - UCLA Human Genetics
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pShuttle <strong>Vector</strong> Map<br />
kanamycin<br />
Pac I<br />
L-ITR<br />
ES<br />
MCS<br />
pShuttle<br />
6.6 kb<br />
right arm homology<br />
pBR322 ori<br />
Pac I<br />
R-ITR<br />
Pme I<br />
left arm homology<br />
pShuttle Multiple Cloning Site Region<br />
(sequence shown 299–459)<br />
Forward primer binding site<br />
GAAGTGAAATCTGAATAATTTTGTGTTACTCATAGCGCGTAATACT...<br />
Kpn I Not I Xho I Xba I EcoR V Hind III Sal I Bgl II<br />
...GGTACCGCGGCCGCCTCGAGTCTAGAGATATCGAATTCAAGCTTGTCGACTCGAAGATCT...<br />
Reverse primer binding site<br />
...GGGCGTGGTTAAGGGTGGGAAAGAATATATAAGGTGGGGGTCTTATGTAGTTTTG<br />
Feature<br />
Nucleotide Position<br />
left Ad5 inverted terminal repeat (ITR) 1–103<br />
encapsidation signal (ES) 183–331<br />
forward primer binding site 299–323<br />
multiple cloning site 345–404<br />
Ad5 right arm homology 414–2652<br />
reverse primer binding site 438–459<br />
Ad5 left arm homology 2701–3580<br />
right Ad5 inverted terminal repeat (ITR) 3581–3683<br />
pBR322 origin of replication 3887–4554<br />
kanamycin resistance ORF 5363–6154<br />
FIGURE 3 The pShuttle vector<br />
12 <strong>AdEasy</strong> <strong>Adenoviral</strong> <strong>Vector</strong> <strong>System</strong>
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