Manual: AdEasy Adenoviral Vector System - UCLA Human Genetics

Guidelines for Infection Conditions and Amplification of the Primary Viral Stock .................. 31
Titer Considerations ............................................................................................................31
Amplification Guidelines .................................................................................................... 31
Infection Procedure Guidelines ........................................................................................... 31
Optimizing Infection Conditions......................................................................................... 32
Monitoring the Infection...................................................................................................... 32
Detection of hrGFP ........................................................................................................................... 32
Monitoring Transfection and Infection of Cells.................................................................. 32
Specifications for hrGFP and EGFP Excitation and Emission Spectra............................... 33
LacZ Control: Detection and Applications..................................................................................... 33
Transfection Control............................................................................................................33
Virus Control....................................................................................................................... 33
Appendix: Plaque Assay using Agarose Overlay ........................................................................... 34
Preparing Viral Stock Dilutions .......................................................................................... 34
Overlaying the Infected Cells with Agarose........................................................................ 34
Plaque Isolation ................................................................................................................... 35
Troubleshooting ................................................................................................................................ 36
Preparation of Media and Reagents................................................................................................ 37
References .......................................................................................................................................... 39
Endnotes............................................................................................................................................. 39
MSDS Information............................................................................................................................ 39