Manual: AdEasy Adenoviral Vector System - UCLA Human Genetics
Manual: AdEasy Adenoviral Vector System - UCLA Human Genetics
Manual: AdEasy Adenoviral Vector System - UCLA Human Genetics
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pShuttle-CMV <strong>Vector</strong> Map<br />
kanamycin<br />
Pac I<br />
L-ITR<br />
ES<br />
P CMV<br />
MCS<br />
SV40 pA<br />
pShuttle-CMV<br />
7.5 kb<br />
pBR322 ori<br />
right arm homology<br />
Pac I<br />
R-ITR<br />
left arm homology<br />
Pme I<br />
pShuttle-CMV Multiple Cloning Site Region<br />
(sequence shown 888–1031)<br />
Forward primer binding site<br />
GGTCTATATAAGCAGAGCTGGTTTAGTGAACCGTCAGATCCGCTAG...<br />
Kpn I Sal I Not I Xho I Hind III<br />
EcoR V<br />
...AGATCTGGTACCGTCGACGCGGCCGCTCGAGCCTAAGCTTCTAGATAAGATATC...<br />
Feature<br />
Reverse primer binding site<br />
...CGATCCACCGGATCTAGATAACTGATCATAATCAGCCATACCAC<br />
left Ad5 inverted terminal repeat (ITR) 1–103<br />
encapsidation signal (ES) 183–331<br />
CMV promoter 341–933<br />
forward primer binding site 888–907<br />
multiple cloning site 940–987<br />
Nucleotide Position<br />
reverse primer binding site 1009–1031<br />
SV40 polyA signal 1011–1238<br />
Ad5 right arm homology 1243–3497<br />
Ad5 left arm homology 3545–4428<br />
right Ad5 inverted terminal repeat (ITR) 4429–4531<br />
pBR322 origin of replication 4735–5402<br />
kanamycin resistance ORF 6211–7002<br />
FIGURE 4 The pShuttle-CMV vector<br />
<strong>AdEasy</strong> <strong>Adenoviral</strong> <strong>Vector</strong> <strong>System</strong> 13
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