Protein A - esonn

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Imaging PIP 2 in vivo using PH-GFP chimeras • PIP 2 is rapidly broken down upon activation of e.g. the angiotensin receptor • A fusion protein PH_GFP, composed of a green fluorescent protein (GFP) and a PH domain binds to PIP 2 in the plasma membrane • Angiotensin addition leads to PIP 2 breakdown => PH-GFP can not bind to the membrane anymore PI(4,5)P 2 specific domain: - [PI(4,5)P 2 ] high in membrane - Angiotensin promotes breakdown Balla, TiPS 2000 14
Domain combination - Variations on a theme “Combinatorial” protein design to create proteins with new properties 15
Page 1 and 2: Advanced biophysics for micro-syste
Page 3 and 4: What does one see in a living cell
Page 5 and 6: Cells - Very complicated reaction c
Page 7 and 8: Molecular interactions - Reductioni
Page 9 and 10: Proteins - Modular assembly of func
Page 11 and 12: Reversible & adjustable protein int
Page 13: Reversible & adjustable protein int
Page 17 and 18: Scaffolds - regulation and integrat
Page 19 and 20: Protein interactions: Genome wide a
Page 21 and 22: Protein interactions: Genome wide a
Page 23 and 24: Interacting molecules - Nanoscopic
Page 25 and 26: Interacting molecules: Monovalent s
Page 27 and 28: Interacting molecules: Immunoglobin
Page 29 and 30: Interacting molecules: Antibody-bin
Page 31 and 32: Interacting molecules: Oligonucleot
Page 33 and 34: Interacting molecules - Nanoscopic
Page 35 and 36: Interacting molecules: LEGO for the
Page 37 and 38: Molecular machines ⇒ Linear motor
Page 43 and 44: Biomolecular nanotechnology => Nano
Page 45 and 46: Where to study the biological objec
Page 47 and 48: Method of choice: Fluorescence What
Page 49 and 50: What kind of molecules to label •
Page 51 and 52: Photoselection and anisotropy of fl
Page 53 and 54: Some basics on fluorescence Fluores
Page 55 and 56: Labeling, with what The probe shoul
Page 57 and 58: Labeling with organic dyes Very lar
Page 59 and 60: Labels: Organic dyes Hard to choose
Page 61 and 62: How to get them • Home made Label
Page 63 and 64: Quantum dots for supra-molecular en
Page 65 and 66:
Reviews • General Marks & Nolan
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Biosynthetic labeling: Fluorescent
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Fluorescent proteins : Switching
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Biosynthetic labeling: Nonnatural a
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Post-biosynthesis labeling: Enzyme
Page 89 and 90:
Post-biosynthesis: Substrate protei
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Post-biosynthesis: Substrate protei
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Post-Biosynthetic labeling: Peptide
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Imaging single 5HT3R receptors usin
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Reversible Sequential single cell l
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Post-Biosynthetic: Chemical labelli
Page 109 and 110:
Post-Biosynthetic: Site-specific si
Page 111 and 112:
Channel gating monitored by fluores
Page 113 and 114:
Reversible vs covalent labeling Cov
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Protein A - esonn

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