Protein A - esonn

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Labeling is needed for identification => Discriminate from matrix and between molecules of interest - Cells are full of fluorescent molecules => Detect the parameter of interest Ideal: • Specific labeling of molecular of interest within a living cell • Stoichiometric • Non-perturbing • Bright and stable labels 48
What kind of molecules to label • Sugars Glycomics – Cell recognition – Protection • Lipids Lipidomics – Membrane barrier – Signaling • Nucleic acids Genomics – Genetic information – Catalysis • <strong>Protein</strong>s Proteomics – Catalysis – Machines • Small molecules Metabolomics Pharmacolomics 49
Page 1 and 2: Advanced biophysics for micro-syste
Page 3 and 4: What does one see in a living cell
Page 5 and 6: Cells - Very complicated reaction c
Page 7 and 8: Molecular interactions - Reductioni
Page 9 and 10: Proteins - Modular assembly of func
Page 11 and 12: Reversible & adjustable protein int
Page 13 and 14: Reversible & adjustable protein int
Page 15 and 16: Domain combination - Variations on
Page 17 and 18: Scaffolds - regulation and integrat
Page 19 and 20: Protein interactions: Genome wide a
Page 21 and 22: Protein interactions: Genome wide a
Page 23 and 24: Interacting molecules - Nanoscopic
Page 25 and 26: Interacting molecules: Monovalent s
Page 27 and 28: Interacting molecules: Immunoglobin
Page 29 and 30: Interacting molecules: Antibody-bin
Page 31 and 32: Interacting molecules: Oligonucleot
Page 33 and 34: Interacting molecules - Nanoscopic
Page 35 and 36: Interacting molecules: LEGO for the
Page 37 and 38: Molecular machines ⇒ Linear motor
Page 43 and 44: Biomolecular nanotechnology => Nano
Page 45 and 46: Where to study the biological objec
Page 47: Method of choice: Fluorescence What
Page 51 and 52: Photoselection and anisotropy of fl
Page 53 and 54: Some basics on fluorescence Fluores
Page 55 and 56: Labeling, with what The probe shoul
Page 57 and 58: Labeling with organic dyes Very lar
Page 59 and 60: Labels: Organic dyes Hard to choose
Page 61 and 62: How to get them • Home made Label
Page 63 and 64: Quantum dots for supra-molecular en
Page 65 and 66: Reviews • General Marks & Nolan
Page 67 and 68: Biosynthetic labeling: Fluorescent
Page 73 and 74: Fluorescent proteins : Switching
Page 79 and 80: Biosynthetic labeling: Nonnatural a
Page 87 and 88: Post-biosynthesis labeling: Enzyme
Page 89 and 90: Post-biosynthesis: Substrate protei
Page 91 and 92: Post-biosynthesis: Substrate protei
Page 93 and 94: Post-Biosynthetic labeling: Peptide
Page 99 and 100:
Post-Biosynthetic labeling: Peptide
Page 101 and 102:
Post-Biosynthetic labeling: Peptide
Page 103 and 104:
Imaging single 5HT3R receptors usin
Page 105 and 106:
Reversible Sequential single cell l
Page 107 and 108:
Post-Biosynthetic: Chemical labelli
Page 109 and 110:
Post-Biosynthetic: Site-specific si
Page 111 and 112:
Channel gating monitored by fluores
Page 113 and 114:
Reversible vs covalent labeling Cov
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Protein A - esonn

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