Protein A - esonn

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Structure of course Part I - Molecules and Labeling • Introduction ÷ Molecular interactions ÷ Molecular machines ÷ Fluorescence • Labeling proteins ÷ During biosynthetic Green fluorescent protein Non-natural amino acids ÷ After biosynthesis Enzyme or substrate fusion Binding partners Chemical reactivity ÷ Covalent vs reversible Part II - Single molecule imaging and spectroscopy • Single molecule detection ÷ Theory ÷ Experiment • Wide-field imaging ÷ Molecular motions ÷ Interactions & catalysis • Confocal detection ÷ Fluctuation analysis ÷ Multi-parameter fluorescence • Nano-devices 2
What does one see in a living cell What changes • Activity • Structure • Location • Concentration • Diffusion • Transport • Interactions 3
Page 1: Advanced biophysics for micro-syste
Page 5 and 6: Cells - Very complicated reaction c
Page 7 and 8: Molecular interactions - Reductioni
Page 9 and 10: Proteins - Modular assembly of func
Page 11 and 12: Reversible & adjustable protein int
Page 13 and 14: Reversible & adjustable protein int
Page 15 and 16: Domain combination - Variations on
Page 17 and 18: Scaffolds - regulation and integrat
Page 19 and 20: Protein interactions: Genome wide a
Page 21 and 22: Protein interactions: Genome wide a
Page 23 and 24: Interacting molecules - Nanoscopic
Page 25 and 26: Interacting molecules: Monovalent s
Page 27 and 28: Interacting molecules: Immunoglobin
Page 29 and 30: Interacting molecules: Antibody-bin
Page 31 and 32: Interacting molecules: Oligonucleot
Page 33 and 34: Interacting molecules - Nanoscopic
Page 35 and 36: Interacting molecules: LEGO for the
Page 37 and 38: Molecular machines ⇒ Linear motor
Page 43 and 44: Biomolecular nanotechnology => Nano
Page 45 and 46: Where to study the biological objec
Page 47 and 48: Method of choice: Fluorescence What
Page 49 and 50: What kind of molecules to label •
Page 51 and 52: Photoselection and anisotropy of fl
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Some basics on fluorescence Fluores
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Labeling, with what The probe shoul
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Labeling with organic dyes Very lar
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Labels: Organic dyes Hard to choose
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How to get them • Home made Label
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Quantum dots for supra-molecular en
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Reviews • General Marks & Nolan
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Biosynthetic labeling: Fluorescent
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Fluorescent proteins : Switching
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Biosynthetic labeling: Nonnatural a
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Post-biosynthesis labeling: Enzyme
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Post-biosynthesis: Substrate protei
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Post-biosynthesis: Substrate protei
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Post-Biosynthetic labeling: Peptide
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Imaging single 5HT3R receptors usin
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Reversible Sequential single cell l
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Post-Biosynthetic: Chemical labelli
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Post-Biosynthetic: Site-specific si
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Channel gating monitored by fluores
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Reversible vs covalent labeling Cov
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Protein A - esonn

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