Protein A - esonn

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Molecular machines ⇒ Linear motors ⇒ Rotational motors ⇒ Energy conversion ⇒ <strong>Protein</strong> factories ⇒ Transport systems ⇒ Channels Bacterial flagella Energy conversion: Electro-chemical >> mechanical Number of H + per revolution: ~ 1000 (~ 6000 kT) Maximum rotation rate: 300 Hz (H + ) or 1700 Hz (Na + ) Maximal speed: 1 µm/s Diameter: 50 nm 38
Molecular machines ⇒ Linear motors ⇒ Rotational motors ⇒ Energy conversion ⇒ <strong>Protein</strong> factories ⇒ Transport systems ⇒ Channels ATPase uses H +- gradient to produce ATP: 3-5 H + needed per ATP Energy conversion: Electro-chemical >> mechanical >> chemical Dimensions: Diameter about 10 nm Rotations: > 3000 rpm => >10’000 ATP/min 39
Page 1 and 2: Advanced biophysics for micro-syste
Page 3 and 4: What does one see in a living cell
Page 5 and 6: Cells - Very complicated reaction c
Page 7 and 8: Molecular interactions - Reductioni
Page 9 and 10: Proteins - Modular assembly of func
Page 11 and 12: Reversible & adjustable protein int
Page 13 and 14: Reversible & adjustable protein int
Page 15 and 16: Domain combination - Variations on
Page 17 and 18: Scaffolds - regulation and integrat
Page 19 and 20: Protein interactions: Genome wide a
Page 21 and 22: Protein interactions: Genome wide a
Page 23 and 24: Interacting molecules - Nanoscopic
Page 25 and 26: Interacting molecules: Monovalent s
Page 27 and 28: Interacting molecules: Immunoglobin
Page 29 and 30: Interacting molecules: Antibody-bin
Page 31 and 32: Interacting molecules: Oligonucleot
Page 33 and 34: Interacting molecules - Nanoscopic
Page 35 and 36: Interacting molecules: LEGO for the
Page 37: Molecular machines ⇒ Linear motor
Page 41 and 42: Molecular machines ⇒ Linear motor
Page 43 and 44: Biomolecular nanotechnology => Nano
Page 45 and 46: Where to study the biological objec
Page 47 and 48: Method of choice: Fluorescence What
Page 49 and 50: What kind of molecules to label •
Page 51 and 52: Photoselection and anisotropy of fl
Page 53 and 54: Some basics on fluorescence Fluores
Page 55 and 56: Labeling, with what The probe shoul
Page 57 and 58: Labeling with organic dyes Very lar
Page 59 and 60: Labels: Organic dyes Hard to choose
Page 61 and 62: How to get them • Home made Label
Page 63 and 64: Quantum dots for supra-molecular en
Page 65 and 66: Reviews • General Marks & Nolan
Page 67 and 68: Biosynthetic labeling: Fluorescent
Page 73 and 74: Fluorescent proteins : Switching
Page 79 and 80: Biosynthetic labeling: Nonnatural a
Page 87 and 88: Post-biosynthesis labeling: Enzyme
Page 89 and 90:
Post-biosynthesis: Substrate protei
Page 91 and 92:
Post-biosynthesis: Substrate protei
Page 93 and 94:
Post-Biosynthetic labeling: Peptide
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Imaging single 5HT3R receptors usin
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Reversible Sequential single cell l
Page 107 and 108:
Post-Biosynthetic: Chemical labelli
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Post-Biosynthetic: Site-specific si
Page 111 and 112:
Channel gating monitored by fluores
Page 113 and 114:
Reversible vs covalent labeling Cov
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Protein A - esonn

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