Counting and measuring using image analysis software
Counting and measuring using image analysis software
Counting and measuring using image analysis software
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<strong>Counting</strong> <strong>and</strong> <strong>measuring</strong><br />
Quick measurements<br />
To make a quick measurement on just one <strong>image</strong><br />
you don’t have to use the ROI Manager. You can just<br />
use the selection tools, draw your selection, <strong>and</strong><br />
click Analyse, Measure<br />
See instructions above on how to change what measurements are displayed (Analyse, Set Measurements)<br />
To remove a selection go to Edit, Selection, Select<br />
None<br />
<strong>Counting</strong> <strong>and</strong> <strong>measuring</strong> <strong>using</strong> Metamorph<br />
• To count the number of cells see the Cell Scoring section of the Metamorph guide; Page 12<br />
Cell scoring uses a maximum of 2 colours, one of which needs to be DAPI nuclei staining.<br />
See also the Nuclei counting section of the Metamorph guide; Page 15, to count just nuclei.<br />
• To count <strong>using</strong> two or more wavelengths, see the Multi-Wavelength cell scoring section of the<br />
Metamorph guide; Page 13<br />
• To measure distance or area in Metamorph you will need to calibrate your <strong>image</strong>s. See the<br />
Calibrating Images section of the Metamorph guide; Page 7<br />
• To make measurements from different regions of interest see the Region Measurements section of<br />
the Metamorph guide; Page 8<br />
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