Invitrogen Bac to Bac Expression System
Invitrogen Bac to Bac Expression System
Invitrogen Bac to Bac Expression System
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The <strong>Bac</strong>-<strong>to</strong>-<strong>Bac</strong> ® <strong>Bac</strong>ulovirus <strong>Expression</strong> <strong>System</strong>, continued<br />
Diagram of the<br />
<strong>Bac</strong>-<strong>to</strong>-<strong>Bac</strong> ®<br />
<strong>System</strong><br />
The figure below depicts the generation of recombinant baculovirus and the<br />
expression of your gene of interest using the <strong>Bac</strong>-<strong>to</strong>-<strong>Bac</strong> ® <strong>Bac</strong>ulovirus <strong>Expression</strong><br />
<strong>System</strong>.<br />
pFast<strong>Bac</strong> donor plasmid<br />
Clone Gene of Interest<br />
Tn7R<br />
P PH<br />
Gene of<br />
Interest<br />
Donor<br />
Tn7L<br />
Transformation<br />
Donor<br />
mini-attTn7<br />
Helper<br />
<strong>Bac</strong>mid<br />
Transposition<br />
Antibiotic Selection<br />
Helper<br />
lacZ<br />
Foreign<br />
Gene<br />
Recombinant<br />
Donor Plasmid<br />
Competent DH10<strong>Bac</strong> E.coli Cells<br />
PPH<br />
E. coli (LacZ - )<br />
Containing Recombinant <strong>Bac</strong>mid<br />
Mini-prep of High<br />
Molecular Weight DNA<br />
or<br />
Determine Viral Titer<br />
by Plaque Assay<br />
Recombinant<br />
<strong>Bac</strong>ulovirus<br />
Particles<br />
Transfection of<br />
Insect Cells with<br />
Cellfectin ® Reagent<br />
Infection of<br />
Insect Cells<br />
Recombinant<br />
<strong>Bac</strong>mid DNA<br />
Recombinant Gene <strong>Expression</strong><br />
or Viral Amplification<br />
5
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