Invitrogen Bac to Bac Expression System
Invitrogen Bac to Bac Expression System
Invitrogen Bac to Bac Expression System
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Map and Features of pFast<strong>Bac</strong> Dual<br />
pFast<strong>Bac</strong> Dual<br />
Map<br />
The map below shows the elements of pFast<strong>Bac</strong> Dual. The complete sequence<br />
of pFast<strong>Bac</strong> Dual is available from our Web site (www.invitrogen.com) or by<br />
contacting Technical Service (see page 62).<br />
Kpn I<br />
Sph I<br />
Nsi I<br />
Pvu II<br />
Nhe I<br />
Nco I<br />
Xho I<br />
Sma I<br />
Bbs I<br />
BamH I<br />
Rsr II<br />
BssH II<br />
EcoR I<br />
Stu I<br />
Sal I<br />
Sst I<br />
Spe I<br />
Not I<br />
Nsp V<br />
Xba I<br />
Pst I<br />
Hind III<br />
P p10<br />
P PH<br />
HSV tk pA<br />
SV40<br />
pA<br />
Tn7L<br />
Gentimicin<br />
pFast<strong>Bac</strong> TM Dual<br />
5238 bp<br />
f1 ori<br />
Comments for pFast<strong>Bac</strong> TM Dual<br />
5238 nucleotides<br />
Tn7R<br />
pUC<br />
f1 origin: bases 102-557<br />
Ampicillin resistance gene: bases 689-1549<br />
pUC origin: bases 1694-2367<br />
Tn7R: bases 2611-2835<br />
Gentamicin resistance gene: bases 2902-3435 (complementary strand)<br />
HSV tk polyadenylation signal: bases 3992-4274 (complementary strand)<br />
Multiple cloning site: bases 4274-4337 (complementary strand)<br />
p10 promoter (P p10 ): bases 4338-4459 (complementary strand)<br />
Polyhedrin promoter (P PH ): bases 4478-4606<br />
Multiple cloning site: bases 4606-4704<br />
SV40 polyadenylation signal: bases 4722-4962<br />
Tn7L: bases 4991-5156<br />
ori<br />
Ampicillin<br />
continued on next page<br />
57
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