Invitrogen Bac to Bac Expression System
Invitrogen Bac to Bac Expression System
Invitrogen Bac to Bac Expression System
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Map of pFast<strong>Bac</strong> 1-Gus<br />
Description<br />
pFast<strong>Bac</strong> 1-Gus is a 6661 bp control vec<strong>to</strong>r containing the Arabidopsis thaliana<br />
gene for β-glucuronidase (Gus) (Kertbundit et al., 1991), and was generated by<br />
restriction cloning of the Gus gene in<strong>to</strong> pFast<strong>Bac</strong> 1. The molecular weight of<br />
β-glucuronidase is 68.5 kDa.<br />
pFast<strong>Bac</strong> 1-Gus<br />
Map<br />
The figure below summarizes the features of the pFast<strong>Bac</strong> 1-Gus vec<strong>to</strong>r. The<br />
complete sequence of pFast<strong>Bac</strong> 1-Gus is available from our Web site<br />
(www.invitrogen.com) or by contacting Technical Service (see page 62).<br />
BamH I<br />
GUS<br />
EcoR I<br />
Stu I<br />
Spe I<br />
Not I<br />
Xba I<br />
Pst I<br />
Xho I<br />
Sph I<br />
Kpn I<br />
Hind III<br />
Gentamicin<br />
P PH<br />
SV40<br />
pA<br />
Tn7L<br />
Tn7R<br />
pFast<strong>Bac</strong> TM 1-Gus<br />
6661 bp<br />
ori<br />
f1<br />
Comments for pFast<strong>Bac</strong> TM 1-Gus<br />
6661 nucleotides<br />
pUC<br />
ori<br />
Ampicillin<br />
f1 origin: bases 2-457<br />
Ampicillin resistance gene: bases 589-1449<br />
pUC origin: bases 1594-2267<br />
Tn7R: bases 2511-2735<br />
Gentamicin resistance gene: bases 2802-3335 (complementary strand)<br />
Polyhedrin promoter (P PH ): bases 3904-4032<br />
GUS ORF: bases 4081-5892<br />
SV40 polyadenylation signal: bases 6047-6287<br />
Tn7L: bases 6315-6480<br />
59
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