Invitrogen Bac to Bac Expression System
Invitrogen Bac to Bac Expression System
Invitrogen Bac to Bac Expression System
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Map of pFast<strong>Bac</strong> Dual-Gus/CAT<br />
Description<br />
pFast<strong>Bac</strong> Dual-Gus/CAT is a 7843 bp control vec<strong>to</strong>r containing the Arabidopsis<br />
thaliana gene for β-glucuronidase (Gus) (Kertbundit et al., 1991) and the<br />
chloramphenicol acetyltransferase (CAT) gene. The vec<strong>to</strong>r was generated by<br />
restriction cloning of the Gus and CAT genes in<strong>to</strong> pFast<strong>Bac</strong> Dual. <strong>Expression</strong> of<br />
CAT and Gus are controlled by the polyhedrin (PH) and p10 promoters,<br />
respectively. The molecular weight of β-glucuronidase and CAT are 68.5 kDa<br />
and 26 kDa, respectively.<br />
pFast<strong>Bac</strong> Dual-<br />
Gus/CAT Map<br />
The figure below summarizes the features of the pFast<strong>Bac</strong> Dual-Gus/CAT<br />
vec<strong>to</strong>r. The complete sequence of pFast<strong>Bac</strong> Dual-Gus/CAT is available from<br />
our Web site (www.invitrogen.com) or by contacting Technical Service (see<br />
page 62).<br />
Kpn I<br />
Sph I<br />
GUS<br />
Xho I<br />
Sma I<br />
Bbs I<br />
BamH I<br />
CAT<br />
Pst I<br />
Hind III<br />
P p10<br />
P PH<br />
HSV tk pA<br />
SV40<br />
pA<br />
Tn7L<br />
Comments for pFast<strong>Bac</strong> TM Dual<br />
5238 nucleotides<br />
pFast<strong>Bac</strong> TM Dual-<br />
Gus/CAT<br />
7843 bp<br />
Polyhedrin promoter (P PH ): bases 16-144<br />
CAT ORF: bases 181-840<br />
SV40 polyadenylation signal: bases 964-1204<br />
Tn7L: bases 4991-5156<br />
f1 origin: bases 1582-2037<br />
Ampicillin resistance gene: bases 2169-3029<br />
pUC origin: bases 3174-3847<br />
Tn7R: bases 4091-4315<br />
Gentamicin resistance gene: bases 4382-4915 (complementary strand)<br />
HSV tk polyadenylation signal: bases 5472-5754 (complementary strand)<br />
GUS ORF: bases 5878-7689 (complementary strand)<br />
p10 promoter (P p10 ): bases 7719-7840 (complementary strand)<br />
Gentimicin<br />
Tn7R<br />
pUC<br />
ori<br />
Ampicillin<br />
f1 ori<br />
61
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