Invitrogen Bac to Bac Expression System

Map and Features of pFastBac 1, continued
Features of the
Vector
pFastBac 1 (4775 bp) contains the following elements. All features have been
functionally tested.
Feature
Polyhedrin promoter (P PH )
Multiple cloning site
SV40 polyadenylation signal
Tn7L and Tn7R
f1 origin
Ampicillin resistance gene
pUC origin
Gentamicin resistance gene
Benefit
Allows efficient, high-level expression of your
recombinant protein in insect cells (O'Reilly et
al., 1992).
Allows restriction enzyme-mediated cloning of
your gene of interest.
Permits efficient transcription termination and
polyadenylation of mRNA (Westwood et al.,
1993).
Mini Tn7 elements that permit site-specific
transposition of the gene of interest into the
baculovirus genome (i.e. bmon14272 bacmid)
(Luckow et al., 1993).
Allows rescue of single-stranded DNA.
Allows selection of the plasmid in E. coli.
Permits high-copy replication and maintenance
in E. coli.
Permits selection of the recombinant bacmid in
DH10Bac E. coli.
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