Invitrogen Bac to Bac Expression System
Invitrogen Bac to Bac Expression System
Invitrogen Bac to Bac Expression System
You also want an ePaper? Increase the reach of your titles
YUMPU automatically turns print PDFs into web optimized ePapers that Google loves.
Product Qualification<br />
pFast<strong>Bac</strong> <br />
Vec<strong>to</strong>rs<br />
1. Each vec<strong>to</strong>r is tested for transposition efficiency in<strong>to</strong> bacmid DNA upon<br />
transformation in<strong>to</strong> MAX Efficiency ® DH10<strong>Bac</strong> competent cells following<br />
the procedure on page 21. Transposition frequency should be greater than 8%<br />
(% white colonies).<br />
2. Restriction analysis with the enzymes below is performed on each vec<strong>to</strong>r <strong>to</strong><br />
confirm its identity. Restriction digests must demonstrate the correct banding<br />
pattern when electrophoresed on an agarose gel.<br />
Vec<strong>to</strong>r Restriction Enzyme Expected Fragments (bp)<br />
pFast<strong>Bac</strong> 1 BamH I<br />
Bgl II<br />
EcoR I<br />
Hind III<br />
Not I<br />
Sal I<br />
4776<br />
470, 4306<br />
4776<br />
4776<br />
4776<br />
4776<br />
pFast<strong>Bac</strong> 1-Gus BamH I<br />
Bgl II<br />
EcoR I<br />
Hind III<br />
Not I<br />
Sal I<br />
6661<br />
2508, 2886<br />
1267, 5394<br />
6661<br />
6661<br />
1886, 4775<br />
pFast<strong>Bac</strong> HT A BamH I<br />
Bgl II<br />
EcoR I<br />
EcoR V<br />
Hind III<br />
Not I<br />
Sal I<br />
4856<br />
1080, 1268, 2508<br />
4856<br />
1263, 3593<br />
4856<br />
4856<br />
4856<br />
pFast<strong>Bac</strong> HT B BamH I<br />
Bgl II<br />
EcoR I<br />
EcoR V<br />
Hind III<br />
Not I<br />
Sal I<br />
4857<br />
470, 4387<br />
4857<br />
404, 1263, 3190<br />
4857<br />
4857<br />
4857<br />
continued on next page<br />
66
Change language