Issue-4_April - Asian Journal of Research in Chemistry (AJRC)

ISSN 0974-4169(print) 0974-4150 (online) www.ajrconline.org Volume 05, Issue 04, April, 2012
RESEARCH ARTICLE
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CONTENT
Quantification of Quercetin and Rutin in Ashwagandharishta by Validated HPTLC Densitometry
Preeti Tiwari and Rakesh K. Patel……………………………………………………………………………………………… 441
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Modulation of Clonazepam Solubility using Solid Dispersion Technique Improves its Solubility and
Bioavailability.
Hanaa A. Fattah, Hammad M. A., Nagia El-Megrab, Waleed Barakat , Ahmed Samir………………………………. 446
•
Use of Activated Carbon of Thevetia peruviana wood for the Adsorption of Acid Violet Dye from Aqueous
Solutions
J. Raffiea Baseri, P.N. Palanisamy and P. Sivakumar……………………………………………………………………… 456
•
Study of Quality Control and Uncertainty in Multiresidue Pesticide Estimation in Packaged Drinking
Water Using GC- MS/MS
S. D. Garway, D. G.Garway, Y. T.Gaikwad, R.M.R. Azad, G.H. Pandya……………………………………………… 462
•
Investigation for bioactive compounds of Aerva lanata (L.) Juss. ex Schultes
A. Anandan, R. Eswaran, A.Doss, G. Sangeetha and S. P. Anand……………………………………………………….. 469
•
RP-HPLC Method Development and Validation for Simultaneous Estimation of Irbesartan and
Hydrochlorothiazide in Pharmaceutical Dosage Form
M.M. Eswarudu, T. Narendra Chary, Sunil Junapudi, M. Sushma. ……………………………………………………… 472
•
A Minuscule Study of Global Warming on Acetobacter aceti-Elevated Temperature and Its Impact over the
Industries
L. Krishnasamy and K. Jayanthi………………………………………………………………………………………………. 477
•
Solubility Enhancement of Poorly Water Soluble Drug by Solid Dispersion Technique
Ahmed Samir, Nagia El-Megrab, Hanaa A. Fattah, Waleed Barakat……………………………………………………. 483
•
The in-vitro Bacteriostatic Potential of Some Traditionally used Indian Spices.
P. Kamleshiya, V.G. Meshram, A.H. Ansari…………………………………………………………………………………. 492
•
Development and validation of UV Spectrophotometric method for quick estimation of milnacipran HCl in
dissolution media.
Nayan C. Ratnakar, K. N. Patel, D. B. Doshi……………………………………………………………………………….. 497
•
Studies of Thermal Conductivity in Carbon Nanotubes Using Simulation
Sunil H. Ganatra, Sneha D. Khobragade, Anushree S. Ujjankar, Chudaman D. Pourkar…………………………… 500
•
Salicylaldehyde Schiff bases bioactivity prediction by Insilico approach
Valli G., Ramu K., Mareeswari P. …………………………………………………………………………………………….. 504
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HPTLC Method for the Simultaneous Estimation of Amlodipine Besylate and Indapamide in Tablet
Formulation
A.K. Desai, R.S. Chauhan, S.A. Shah, D.R. Shah…………………………………………………………………………… 510
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Validated RP-HPLC Method for Estimation of Rasagiline in Tablet Dosage Form
Bhavin Vaishnani, Ritu Kimbahune, Prachi Kabra, Sanjay Surani, L.V.G. Nargund…………………………………. 515

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Surfactants as Corrosion Inhibitors for Carbon Steel in Hydrochloric acid Solution
Diwan Singh Rajan and Dr. Shiv Darshan Malik…………………………………………………………………………… 520
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UV-Spectrophotometric Determination of Acephate and Profenofos (organophosphate) Pesticides in Buffer
Medium
N.V.S. Venugopal and B. Sumalatha………………………………………………………………………………………….. 526
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Design, Synthesis and Physico-Chemical Investigation of the Iron (II) Complexes Containing Heterocyclic
Nitrogen Donor Ligands
S.K. Khatua, S.C. Nayak, P.K.Das, R.R. Guin………………………………………………………………………………. 529
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Design, Synthesis and Characterization of Some Novel Amino and Aldehyde Substituted
Azaphenothiazines
Dhiraj Raghuvanshi, Pramod Kumar Sharma, Nimita Manocha, Shikha Agrawal, Prerna Chaturvedi……………. 532
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Ion-Pair Spectrophotometric Estimation of Ciprofloxacin in bulk and pharmaceutical formulations
A. S. Grewal, S. K. Patro, S. K. Kanungo……………………………………………………………………………………. 537
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Removal of Chromium (III) from drinking water using ash of bark of Terminalia arjuna
M.N.Thakuria and A. K. Talukdar…………………………………………………………………………………………….. 541
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Synthesis and Characterization of Some Complexes of Potassium (I) With 1,4,7,10,13,16-
Hexaoxacyclooctadecane
Rajeev Ranjan and Ramjatan Sinha…………………………………………………………………………………………. 547
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Equilibrium Dialysis Studies on the Interaction of some Surfactants with Poly(N-vinyl-2-pyrrolidone) and
Bovine Serum Albumin
Ashok Kumar, O.P. Yadav……………………………………………………………………………………………………… 552
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Comparison Study of CNS Depressant Study of Synthesized Schiff Bases of Mannich Bases of
Resacetophenone Having Variable Electronegative Atoms (:O:/:S:/:NH)
Arpit D. Shah, Devanshi J. Raval, Nisha D. Pandya, Sunita J. Panchal, Viraj P. Jatakiya and Dr. Dhrubo Jyoti Sen……. 556
REVIEW ARTICLE
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Liquid Crystal- a Review
Abhijit Ray…………………………………………………………………………………………………………………………. 563
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Instruction to author .................................................................................................................................................. 568

Abstract
RESEARCH ARTICLE
Quantification of Quercetin and Rutin in Ashwagandharishta by Validated HPTLC Densitometry
Preeti Tiwari 1 * and Rakesh K. Patel 2 ……………………………………………………………………………………….441
1 Department of Pharmacognosy, Shri Sarvajanik Pharmacy College, Mehsana-384001, India.
2 Head of Department of Pharmacognosy, Shri S. K. Patel College of Pharmaceutical Education and Research,
Kherva-382711, India
ABSTRACT:
Ashwagandharishta is a polyherbal hydro-alcoholic formulation and is used as immunomodulator to promote the
health and longevity by increasing defence against disease and also known for its usefulness in the treatment of
hypercholesterolemia. A simple, precise and accurate HPTLC method has been established for the determination of
quercetin and rutin in Ashwagandharishta–T and Ashwagandharishta-M prepared by traditional and modern
methods respectively and also in its marketed formulation. The developed HPTLC method was validated in terms of
precision, accuracy, LOD, LOQ and specificity. The amount of quercetin in Ashwagandharishta-T, M and its
marketed formulation was found to be 0.0021, 0.00192 and 0.00197 % w/w respectively while rutin was found to be
0.00469, 0.00441 and 0.00464 % w/w respectively. This is the first report for the quantification of quercetin and
rutin in Ashwagandharishta by HPTLC. Furthermore, no TLC densitometric methods have been reported for the
quantification of quercetin and rutin from Ashwagandharishta.
Modulation of Clonazepam Solubility using Solid Dispersion Technique Improves its Solubility and
Bioavailability.
Hanaa A. Fattah 1 , Hammad M. A. 1 , Nagia El-Megrab 1 , Waleed Barakat 2 , Ahmed Samir 1 ………………………446
1 Department of Pharmaceutics, Faculty of Pharmacy, Zagazig University, Egypt.
2 Department of Pharmacology, Faculty of Pharmacy, Zagazig University, Egypt.
ABSTRACT:
The pharmacologic efficacy of benzodiazepines (as clonazepam, CZP) in controlling seizures is directly associated
with their plasma concentration. An emergent epileptic situation necessitates rapid drug absorption. parentral
administration is not convenient in epileptic emergency and oral bioavailability of CZP is limited by its poor water
solubility.
Solid dispersion formulations were developed by physical mixture and solvent evaporation using CZP with the
carriers polyvinyl pyrrolidone K30 (PVP K30, 1:4) or polyethylene glycol 4000 (PEG 4000, 1:4).
Analysis of the solid dispersions by FTIR, DSC, XRD and dissolution studies showed that CZP was molecularly
dispersed in an amorphous form and indicate increased dissolution rate.
In addition, the oral bioavailability of the formulations was significantly improved reaching therapeutic levels within
30 and 60 min in case of PEG 4000 and PVP K30 respectively following oral administration to mice.
In conclusion; PVP K 30 and PEG 4000 can be utilized to increase the solubility of CZP and enhance its oral
bioavailability.
Use of Activated Carbon of Thevetia peruviana wood for the Adsorption of Acid Violet Dye from Aqueous
Solutions
J. Raffiea Baseri 1 , P.N. Palanisamy 2 and P. Sivakumar 3 ………………………………………………………………..456
1 Department of Chemistry, Al-Ameen Engineering College, Erode, Tamilnadu-638 104, India
2 Department of Chemistry, Kongu Engineering College, Perundurai, Erode, Tamilnadu- 638 052,India
3 Department of Chemistry, Arignar Anna Government Arts College, Namakkal, Tamilnadu- 637 002, India

ABSTRACT:
Activated carbon prepared from Thevetia peruviana wood was used as an adsorbent for the removal of Acid Violet
49 from aqueous solutions. Batch adsorption experiments were performed for adsorption kinetics and isotherm by
varying temperature, initial dye concentration, pH and time. The amount of dye adsorbed increased while increasing
the initial concentration and temperature of the solution. The experimental data fitted well to Pseudo second order
kinetic model with Intra particle diffusion as the rate determining step. Adsorption data fitted with Langmuir model.
The negative value of Gibb’s free energy (G°) indicated that the adsorption was spontaneous in nature. The results
showed that the activated carbon of Thevetia peruviana was suitable for the removal of the acidic dyes from the
aqueous solutions. The positive value of enthalpy ( ) indicated that the adsorption of the selected dyes were
endothermic.
KEYWORDS : Thevetia Peruviana, Adsorption, Dyes, Acid Violet 49, kinetics, Activated carbon.
Study of Quality Control and Uncertainty in Multiresidue Pesticide Estimation in Packaged Drinking Water
Using GC- MS/MS
S. D. Garway, D. G.Garway, Y. T.Gaikwad, R.M.R. Azad, G.H. Pandya*……………………………………………462
Research and Development Division, Anacon Laboratories Pvt. Ltd., F-34,35, MIDC, Butibori, Nagpur 441122, India
ABSTRACT:
A method was developed and validated for the simultaneous determination of 15 Organochlor pesticides residues in
packaged drinking water by gas chromatography coupled to a triple quadrupole mass analyzer (GC/-MS/MS),
mainly using the selected reaction monitoring (SRM) mode. The recovery data were obtained by spiking blank
samples of packaged drinking water at concentration levels of 0.09 g/L, yielding recoveries in the range 70–110%.
Precision values expressed as relative standard deviation (RSD) were in the range of 4.7 -18.4 %. Linearity was
studied in the range 10–200ng/L and the coefficient of correlation was higher than 0.98% for all compounds.
Method Detection Limits (MDLs) and limits of quantification (LOQs) were established. The overall uncertainty of
the method was estimated. According to the validation data and performance characteristics as well as the high
sample throughput, the proposed method is suitable for routine application.
KEYWORDS : Pesticides, Packaged water, GC-MS/MS, Uncertainty.
Investigation for bioactive compounds of Aerva lanata (L.) Juss. ex Schultes
A. Anandan 1 , R. Eswaran 1 , A.Doss 2 , G. Sangeetha 3 and S. P. Anand 2* ………………………………………………469
1 Department of Genetics and Plant Breeding, Annamalai University, Annamali Nagar - 608 002, Tamil Nadu, India
2 PG & Research Department of Botany, National College (Autonomous), Tiruchirappalli - 620 001, Tamil Nadu, India
3 Department of Plant Pathology, Annamalai University, Annamali Nagar - 608 002, Tamil Nadu, India,
ABSTRACT:
Aerva lanata commonly known as “Sunny khur” is widely used in Indian folk medicine for the treatment of various
infectious diseases. This study was undertaken to analyze the various bioactive compounds of alcoholic extract of
A.lanata using Chromatographic techniques. In this study, fatty acid esters, fatty acid amide, triterpene, diterpene
alcohols and phytol were identified as the major chemical groups in the methanol fractions of A.lanata leaf extracts.
Their structures were elucidated, on the basis of GC-MS data, D:B-Friedo-B’:A’-neogammacer-5-en-3-ol (3 )-
(33.60%), Myo-Inositol,4-C-methyl- (26.67%), Benzoic acid, 3,5-bis(1,1-dimehtylethyl)-4-hydroxy- (18.93%), 1,2-
Benzenedicarboxylic acid, bis(2-methylpropyl) ester (7.73%), n-Hexadecanoic acid (7.73%), Phytol (1.87%) and
Hexadecanoic acid, ethyl ester (1.60%). The results obtained fully indicated that the alcohol extract of A.lanata
leaves had the great potential of noble natural medicines.
KEYWORDS: GC-MS, Fatty acids, Phytol, Methanol, titerpene
RP-HPLC Method Development and Validation for Simultaneous Estimation of Irbesartan and
Hydrochlorothiazide in Pharmaceutical Dosage Form
M.M. Eswarudu 1* , T. Narendra Chary 1 , Sunil Junapudi 1 , M. Sushma 2 . …………………………………………….472

1. Department of pharmaceutical analysis, Anurag pharmacy college, Ananthagiri(V), Kodad(M), Nalgonda (Dt),
Andhra Pradesh, India.508206
2. D.C.R.M. Pharmacy College, Inkollu, Prakasham Dist, Andhra Pradesh, India.
ABSTRACT:
A simple, reproducible and efficient high performance liquid chromatographic method was developed for
simultaneous determination of Irbesartan and Hydrochlorothiazide in tablets. A Hypersil pack BDS C18 column
250X4.6 mm 5µ particle size in isocratic mode with mobile phase containing acetonitrile: Buffer (sodium acetate
anhydrous) (55:45 v/v ) adjusted to pH 3.5 using ortho phosphoric acid. The flow rate was 1.0 ml/min and effluent
was monitored at 260 nm. The retention time and linearity range for Irbesartan and Hydrochlorothiazide were (2.98,
4.83 min) and (5-200, 1-400 g/ml), respectively. The developed method was found to be accurate, precise and
selective for simultaneous determination of Irbesartan and Hydrochlorothiazide in tablet dosage form.
KEYWORDS: Irbesartan, Hydrochlorothiazide, RP-HPLC, simultaneous determination, Validation.
A Minuscule Study of Global Warming on Acetobacter aceti-Elevated Temperature and Its Impact over the
Industries
L. Krishnasamy and K. Jayanthi……………………………………………………………………………………………..477
Dept. of Biotechnology, Hindustan College of arts and science, Kellambakkam-603 103, Chennai, Tamil Nadu
ABSTRACT:
The aim of this study was to investigate the effect elevated temperature on A.aceti which converts ethanol to acetic
acid in the presence of oxygen. A.aceti was incubated at various temperatures like 37°C, 42°C, and 47°C for
overnight. After exposure, the DNA content in each culture was determined. The results showed that an increase in
temperature like 42°C and 47°C produced a marked decrease in the DNA content when compared to the culture
incubated at 37°C. The expression of gene was confirmed using PCR. The PCR result showed that the gene citrate
synthase of A.aceti was expressed in the culture incubated at 37°C and a significant suppression of the gene citrate
synthase was seen in the culture treated at 42°C and 47°C.Therefore the elevated temperature on A.aceti will
significantly affect the various importance of the organism especially in vinegar production where A.aceti plays a
vital role.
KEYWORDS: A.aceti, elevated temperature, vinegar, citrate synthase gene
Solubility Enhancement of Poorly Water Soluble Drug by Solid Dispersion Technique
Ahmed Samir 1 , Nagia El-Megrab 1 , Hanaa A. Fattah 1 , Waleed Barakat 2 ……………………………………………483
Departments of 1 Pharmaceutics, 2 Pharmacology, Faculty of Pharmacy, Zagazig University, Egypt.
ABSTRACT:
The objective of present was to improve the solubility of Clonazepam (CZP), poorly water soluble drug, by solid
dispersion technique using Polyethylene glycol 6000 (PEG 6000) and Urea (UR) as carriers. The Solid dispersion
was prepared by physical mixing and solvent evaporation method. The interaction of the Clonazepam with PEG
6000 and UR was evaluated by the Fourier transform infrared (FTIR) spectroscopy; Differential scanning
Calorimetry (DSC) and X-ray diffraction patterns (XRD). The results from the FTIR and XRD analyses showed that
Solid dispersion might exist in the amorphous form. A DSC result showed that the sharp melting point was
completely disappeared suggesting that the CZP molecularly dispersed in an amorphous form. Dissolution studies
indicate that dissolution rate was remarkably increased in Solid dispersion as compared to the physical mixture and
drug alone. Also the pharmacological studies of the selected formulations [(1:3 CZP : PEG 6000) and (1:2 CZP :
Urea)] were performed. In conclusion PEG 6000 and UR can be a well utilized to increase the solubility of poorly
water soluble drugs.
KEY WORDS: Clonazepam, solid dispersion, Polyethylene glycol 6000, Urea.

The in-vitro Bacteriostatic Potential of Some Traditionally used Indian Spices.
P. Kamleshiya 1 *, V.G. Meshram 1 , A.H. Ansari 2 ………………………………………………………………………….492
1 P.G. Department of Biochemistry, RTM Nagpur University LIT Campus Nagpur , India
2 Department of Biochemistry, S .K. Porwal College, Kamthee, Nagpur India
ABSTRACT:
The in-vitro antibacterial activity of aqueous and methanolic extract of some Indian spices, Cinnamom cassia,
Myristica fragrans, Murraya koenigii, Piper nigrum and Trachyspermum ammi against Bacillus Subtilis, Bacillus
Cereus, Escherichia Coli, Psuedomonas aeruginosa and Staphylococcus aureus was evaluated by using agar
diffusion assay and their antibacterial effect was compared with some standard antibiotics. The minimum inhibitory
concentration (MIC) of the extracts showing higher efficacy against the test organism was determined. By and large,
all the extract possessed antimicrobial property but comparatively the methanolic extracts are more potent than
aqueous extract of spices. MICs for aqueous and methanolic extracts ranged from 20-80 mg/ml and 5-20 mg/ml
respectively. The obtained results shows that spices have a natural bacteriostatic potential to be used as antimicrobial
agent.
KEYWORDS: Spice, Antibacterial activity, aqueous extract and methanolic extract.
Development and validation of UV Spectrophotometric method for quick estimation of milnacipran HCl in
dissolution media.
Nayan C. Ratnakar 1 *, K. N. Patel 2 , D. B. Doshi 3 …………………………………………………………………………497
1 Research Scholar, Singhania University, Jhunjhunu, Rajasthan.
2 SAL Institute of Pharmacy, Ahmedabad.Gujarat.
3 L. M. College of Pharmacy, Ahmedabad, Gujarat.
ABSTRACT:
A simple, sensitive, accurate, precise, reproducible, economic spectrophotometric method has been developed for
the estimation of milnacipran hydrochloride in dissolution medium. UV spectrophotometric method is based on
measurement of absorption at maximum wavelength 223 nm. The percentage recovery of milnacipran HCl ranged
from 99.82-101.23 %.in pharmaceutical dosage form. The developed method was validated with respect to linearity,
accuracy (recovery), precision (intra and interday variations). and specificity. Beers law was obeyed in the
concentration range of 2-40g/ml with correlation coefficient of 0.997. Results of the analysis were validated
statistically and by recovery study.
KEYWORDS: Milnacipran HCl; Spectrophotometric analysis; Validation; precision; accuracy; LOD; LOQ.
Studies of Thermal Conductivity in Carbon Nanotubes Using Simulation
Sunil H. Ganatra*, Sneha D. Khobragade, Anushree S. Ujjankar, Chudaman D. Pourkar……………………...500
Department of Chemistry, Institute of Science, R. T. Road, Nagpur – 440001, M.S. India
ABSTRACT:
The Nano Heat flow tool allows users to explore the time evolution of kinetic and potential energy among the
vibrational modes of a carbon nanotube over the course of a molecular dynamics (MD) simulation. It is possible to
observe the drop of vibrational energy through the modes of the system as a non-equilibrium population of phonons
is dissipated towards thermal equilibrium, and thus gives insight into the basic sources of damping and dissipation
within nanoscale objects.
Molecular Dynamics Simulation for Heat Flow in C(6,0), C(6,3) and C(6,6) carbon nanotubes were studied at
289.15 K using high performance parallel processing computers.
It is reported that the selected nanotubes i.e. C(6,0), C(6,3) and C(6,6) shows the thermal conductivity at various
frequencies.
The surprise results for C(6,0) and C(6,6) where the frequency is highly selective and give single peak. Whereas, in
case of C(6,3) various frequency peaks are reported and spread in wide spectrum.

KEYWORDS: Carbon nanotubes, Heat Flow, Thermal conductivity, Molecular dynamics.
Salicylaldehyde Schiff bases bioactivity prediction by Insilico approach
Valli G., Ramu K., Mareeswari P. …………………………………………………………………………………………..504
Department of Chemistry, SFR College for women, Sivakasi.
ABSTRACT:
Our study focuses the prediction of the bioactivity of Salicylaldehyde Schiff bases by computational method using
PASS (online software for bioactivity prediction) by knowing the importance of Schiff bases derived from
salicylaldehyde with various amines. Thirteen Schiff bases selected for our work. PASS prediction result showed
that salicylaldehyde o-phenylenediamine Schiff bases possessed higher laccase inhibitor (91.6% )activity and 3-
hydroxybenzoate 4-monooxygenase inhibitor(88%) activities. salicylaldehyde luicine Schiff base possessed
antiseborrheic(86.1%), Pro-opio melanocortin converting enzyme inhibitor(86.5%) and protein-glutamate methyl
esterase activity(85.2%). salicylaldehyde Schiff bases derived from 2-aminopyridine, ethylenediamine, Glycine, 2,4-
dinitrophenylhydrazine, 4-aminobenzene sulphonamide and methionine were found to have Glutathione
thiolesterase inhibitor(86.3%), Glucose oxidase inhibitor (86.1%), Monophenol monooxygenase inhibitor (86.5%) ,
Cis-1,2-dihydro-1,2dihydroxy naphthalene dehydrogenase inhibitor (89.5%),Anthranilate 3-monooxygenase
(deaminating)Inhibitor(88.7%) ,Corticosteroid side-chain-isomerase inhibitor (88.7%), Sulfite reductase
inhibitor(86.1%), Pullulanase inhibitor (85.8%) and 3-Phytase inhibitor(85.4%). Aryl acetonitrilase inhibitor
(85.2%), Arylformamidase inhibitor (91.2%), Phenylalanine (histidine) transaminase inhibitor (89.4%) and L-3-
cyanoalanine synthase inhibitor (88.9%) activities. They also found to act as Dopamine D4 agonist (89.5%) and
Antiprotozoal (Toxoplasma) (87%) compared to other Schiff bases.
KEYWORDS: o-phenylenediamine, Luicine, Laccase , salicylaldehyde, PASS.
HPTLC Method for the Simultaneous Estimation of Amlodipine Besylate and Indapamide in Tablet
Formulation
A.K. Desai*, R.S. Chauhan, S.A. Shah, D.R. Shah………………………………………………………………………..510
Maliba Pharmacy College, Bardoli, Gujarat, India -394350.
ABSTRACT:
A simple, precise and accurate high performance thin layer chromatographic method was developed and validated
for the simultaneous estimation of amlodipine besylate and indapamide in combined tablet dosage form. Pre-coated
silica gel 60F254 aluminium plate was selected as the stationary phase and dichloromethane: methanol: ammonia
8.5: 1.5: 0.1 (v/v/v) was used as developing mobile phase. The detection of amlodipine and indapamide was carried
out at 241nm. The method was validated for linearity, accuracy, precision, limit of detection and limit of
quantitation parameters. The correlation coefficient of amlodipine and indapamide were found to be 0.9995 and
0.9977 respectively. The average percentage recovery of amlodipine and indapamide were 98.49 -102.05 and 99.2 -
102.01 respectively. The proposed HPTLC method has potential applications for determination of amlodipine
besylate and indapamide in combined tablet dosage form.
KEYWORDS: HPTLC method, amlodipine, indapamide, method development, validation.
Validated RP-HPLC Method for Estimation of Rasagiline in Tablet Dosage Form
Bhavin Vaishnani, Ritu Kimbahune, Prachi Kabra*, Sanjay Surani, L.V.G. Nargund…………………………….515
Department of Quality Assurance, Nargund College of Pharmacy, Dattatreyanagar, II Main,100 Ft Ring Road, BSK
III Stage, Bangalore- 560 085, India.
ABSTRACT:
In the present study, a reverse phase high performance liquid chromatographic method was developed and validated
for the determination of Rasagiline in tablet dosage form with the use of Caffeine as an internal standard.

Chromatographic separation was carried out on a RP-18 column using a mobile phase consisting of acetonitrile:
isopropyl alcohol: water (10:20:70, v/v/v) adjusted at pH 2.5 with o-phosphoric acid (1%). The flow rate was
maintained at 0.8 ml min−1 and UV detection was measured at 210 nm. The calibration curve was linear over the
range 1-200µgml−1. R.S.D. for precision was

ABSTRACT:
A number of iron metal complexes of type [FeL 4 (H 2 O) 2 ]SO 4 where L=hetero cyclic nitrogen donor ligands
(imidazole, pyridine, piperadine piperazine, indole, benzimidazole, benzotriazole, triazole, quinoline, iso-quinoline,
carbazole) have been synthesized and characterized. These complexes are soluble in ordinary organic solvent. The
conductivity data shows that these complexes are 1:1 electrolytic in nature. The IR spectra reveal the coordination of
nitrogen of the ligand to central iron metal atom. The mass spectra for benzimidazole complex shows the molecular
ion peak at 660 and base peak at 119. The thermal decomposition studies indicate the presence of water molecule
which is associated to central metal ion. The loss of water molecule and decomposition of sulphate ion occurs at
250 0 c. Then after 100 0 c imidazole molecule is removed one by one, giving the Fe 3 O 4 as the final product.
KEYWORDS: Metal, complexes, ligands, indole, benzimidazole, triazole, IRspectra, mass spectra.
Design, Synthesis and Characterization of Some Novel Amino and Aldehyde Substituted Azaphenothiazines
Dhiraj Raghuvanshi 1 *, Pramod Kumar Sharma 1 , Nimita Manocha 1 , Shikha Agrawal 2 , Prerna
Chaturvedi 1 ……………………………………………………………………………………………………………………….532
1 Department of Pharmaceutical Chemistry, Swami Vivekanand College of Pharmacy, Near Toll Naka, Khandwa Road,
Indore, M.P. PIN-452020
2 Department of Pharmaceutics, Swami Vivekanand College of Pharmacy, Near Toll Naka, Khandwa Road, Indore, M.P.
PIN-452020
ABSTRACT:
Some novel series of amino and aldehyde substituted azaphenothiazines were synthesized by condensation of 2-
chloro-1-(10H-azaphenothiazin-10yl)ethanone with various amines and aryl aldehydes. As it is known that
substituted aldehyde and amine when attached with some alkyl chain or heterocyclic moiety can lead to potentially
biological active compound. The structure modification for their better biological activity includes substitution on
nitrogen at 10th position coupled with aliphatic/aromatic amine and aromatic aldehydes. The above result
established the fact that azaphenothiazine can be a rich source of biologically active compounds. The synthesized
compounds were confirmed with IR, 1HNMR and Mass spectroscopy. The bioavailability of compounds was
determined by Lipinski rule of 5 using customized software.
KEYWORDS: Azaphenothiazine, Bioavailability, Synthesis, Amino, Aryl, Mobile phase.
Ion-Pair Spectrophotometric Estimation of Ciprofloxacin in bulk and pharmaceutical formulations
A. S. Grewal 1* , S. K. Patro 2 , S. K. Kanungo 2 ……………………………………………………………………………..537
1 Jan Nayak Ch. Devi Lal Memorial College of Pharmacy, Sirsa 125055, Haryana, India
2 Institute of Pharmacy and Technology, Salipur, Cuttack 754202, Orissa, India
ABSTRACT:
The main objective was to develop and validate asimple, accurate, precise and sensitive ion-pair spectrophotometric
extraction method for the assay of Ciprofloxacin (CFX) in pure drug and tablets. The method is based upon the
reaction of Ciprofloxacin with methyl orange, forming a yellow colour complex in acidic medium, which is
extracted in chloroform and analyzed. The extracted complexes showed absorbance maxima (max) found to be at
429 nm. Beer's law was obeyed for a wide concentration range i.e.100-700 g/mLas the extracted species seemed
well defined and stable.Surface or an interphase adsorption phenomenon was not a problem. Optimization of the
reaction was carried out with factors such as buffer strength, stability of complex, molar ratio of drug: dye and
extraction time.The proposed method was validated as per ICH guidelines Q 2. The recovery studies confirmed the
accuracy and precision of the method. The above method was a rapid tool for routine analysis of Ciprofloxacin in
the bulk and pharmaceutical dosage forms.
KEYWORDS: Antibiotic; Ciprofloxacin determination; spectrophotometry; ion pair extraction; fluoroquinolone
Removal of Chromium (III) from drinking water using ash of bark of Terminalia arjuna

M.N.Thakuria 1 and A. K. Talukdar 2 …………………………………………………………………………………………541
1 Department of Chemistry, Birjhora Mahavidyalaya, Bongaigaon -783380, Assam, India
2 Department of Chemistry, Gauhati University, Guwahati-781014, Assam, India
ABSTRACT:
Drinking water samples of 27 sources of Chirang District of Assam, India have been analyzed. Ash bark of
Terminalia arjuna was studied as adsorbent for adsorption of Cr 3+ ions from contaminated water based on these
findings. Terminalia arjuna was characterized by X ray fluoresence spectroscopy for determining chemical
composition, X ray diffraction to verify crystallinity, fourier transform infra red spectroscopy for residual organic
species and N 2 adsorption studies to find out surface area and pore volume. The adsorption experiments were
applied to evaluate the adsorption performances of Terminalia arjuna. The maximum (99%) removal of Cr (III) can
be obtained if 60 mL of water containing 0.8g L -1 of Cr (III) was passed through 5 g of Terminalia arjuna bark ash
for 2 elutions. Langmuir’s and Freundlich’s adsorption isotherms gave satisfactory fitting to the adsorption isotherm
data.
KEYWORDS: Terminalia arjuna; Cr (III); Adsorption; Drinking water; Chirang District
Synthesis and Characterization of Some Complexes of Potassium (I) With 1,4,7,10,13,16-
Hexaoxacyclooctadecane
Rajeev Ranjan 1 and Ramjatan Sinha 2 ……………………………………………………………………………………..547
1 PG Department of Chemistry, Ranchi College, Ranchi-834008
2 University Department of Chemistry, Patna University, Patna-800005
ABSTRACT:
The present paper describes the preparation and characterization of some potassium ion complexes with
1,4,7,10,13,16,-hexaoxacyclooctadecane having six donar oxygen atoms. The potassium metal compounds used for
complexation are salts of nitrophenols and 8- hydroxyquinoline. Products were isolated from potassium salt of all
the four monoionic ligands, 2-nitrophenol (ONPH), 2,4-dinitrophenol (DNPH), 2,4,6-trinitrophenol (TNPH) and 8-
hydroxyquinoline (8HQH) with 18-crown-6 ether. It was found that potassium salts and 18-crown-6 ether did bind
together, rather than they remain uninteracted. The products in each procedure was chromotographed using TLC.
The TLC products were used to identify the interaction and formation of stable compounds. The bonding pattern and
structure of complexes were suggested from the studies of elemental analysis, IR, UV and 1 H-NMR spectral
analysis.
KEYWORDS: 18-CROWN-6, TNPH, 8-HQH
Equilibrium Dialysis Studies on the Interaction of some Surfactants with Poly(N-vinyl-2-pyrrolidone) and
Bovine Serum Albumin
Ashok Kumar, O.P. Yadav*…………………………………………………………………………………………………..552
Department of Chemistry, CCS Haryana Agricultural University, Hisar-125001, India
ABSTRACT:
Inter-molecular interactions of sodium dodecylbenzene sulphonate (SDBS) and cetyl-trimethyl ammonium bromide
(CTAB) with poly-(N-vinyl-2-pyrrolidone) (PVP) and bovine serum albumin (BSA) have been studied at 308.15K
from equilibrium dialysis measurements. The process of binding of SDBS with PVP is found to complete through
three distinct stages marked as (i) a weak interaction region, (ii) a strong cooperative binding region and (iii) a no
binding region. Stronger interaction of anionic surfactant (SDBS) with BSA compared to DTAB, suggests the
existence of positively charged binding sites at PVP molecule. The observed surfactant-polymer binding data have
been analysed in the light of various theoretical models for ligand-macromolecule interactions. The surfactant
molecules bind with the macromolecule as monomer, in a co-operative manner.
KEYWORDS: dialysis, equilibrium, interactions, macromolecules, polymer, surfactant

Comparison Study of CNS Depressant Study of Synthesized Schiff Bases of Mannich Bases of
Resacetophenone Having Variable Electronegative Atoms (:O:/:S:/:NH)
Arpit D. Shah, Devanshi J. Raval, Nisha D. Pandya, Sunita J. Panchal, Viraj P. Jatakiya and Dr. Dhrubo Jyoti
Sen…………………………………………………………………………………………………………………………………556
Department of Pharmaceutical Chemistry, Shri Sarvajanik Pharmacy College, Gujarat Technological
University, Arvind Baug, Mehsana-384001, Gujarat, India
ABSTRACT:
Three Schiff bases of Mannich bases of resacetophenone have been synthesised by using three variable
electronegative atoms (X=O/S/NH) of urea/thiourea/guanidine and tested for CNS depressant activity. It has been
found that Compound-3c (X=NH) showed maximum CNS depressant effect in the Albino mice and producing more
sedative effect and more sleeping time, Compound-3a (X=O) showed less sleeping time than guanidine derivative.
There is very less effect compared to both very less sedative effect in the case of Compound-3b (X=S). CNS
depressant activity profile of three compounds is as follows: Compound: 3c (X=NH) > 3a (X=O) > 3b (X=S). LogP
values of three compounds were found to be X=O: 4.03, X=S: 4.63 and X=NH: 3.79. So, the partition coefficient is
in this order: X=S: 4.63 > X=O: 4.03 > X=NH: 3.79. Partition coefficient of X=S is 4.63 which is greater than X=O
(4.03) and X=NH (3.79). Electronegativity of S is 2.58 which is in between O=3.44 and NH=3.04. Electronegative
atoms O and S both have two lone pair of electrons but electronegativity for O: 3.44 > N: 3.04 > S: 2.58. The
electronegativity of N is 3.04 so the NH compound (guanidine) showed maximum CNS depression,
electronegativity of O is 3.44 so the O compound (urea) showed medium CNS depression and the electronegativity of
S is 2.58 so the S compound (thiourea) showed minimum CNS depression. The log P values of NH compound
(guanidine) is 3.79 which is minimum but showed maximum CNS depression, log P value of O compound (urea) is
4.03 which showed medium CNS depression and log P value of S compound (thiourea) is 4.63 which showed
minimum CNS depression. Oxygen and Sulfur both have two lone pairs of electrons so the CNS depression has been
found lesser than NH group which has one lone pair of electrons and the potency of CNS depression is due to the
difference in electronegativity: O: 3.44, S: 2.58 and NH: 3.79.
KEYWORDS: Schiff base, Mannich base, cut off, sleeping time.
REVIEW ARTICLE
Liquid Crystal- A Review
Abhijit Ray……………………………………………………………………………………………………………………….563
HOD, Department of Biotechnology, Raipur Institute of Technology, Raipur (CG)
ABSTRACT:
Liquid crystal has tremendous impact in our day to day life. Many substances can exist in more than one state. For
example, water can exist as a solid (ice), liquid, or gas(water vapor). The state of water depends on its temperature.
Below 0 0 C, water is a solid. As the temperature rises above 0 0 C, ice melts to liquid water. When the temperature
rises above 100 0 C, liquid water vaporizes completely. Some substances can exist in states other than solid, liquid,
and vapor. For example, cholesterol myristate (a derivativeof cholesterol) is a crystalline solid below 71 0 C. When
the solid is warmed to 71 0 C, it turns into a cloudy liquid. When the cloudy liquid is heated to 86 0 C, it becomes a
clear liquid. Cholesterol myristate changes from the solid state to an intermediate state (cloudy liquid) at 71 0 C, and
from the intermediate state to the liquid state at 86 0 C. Because the intermediate state exits between the crystalline
solid state and the liquid state, it has been called the liquid crystal state. Thus Liquid crystals (LC) are a state of
matter that has the property between those of a conventional liquid and those of solid crystal.
KEYWORDS: liquid crystals, LC, mesogen, thermotrophic, LCD