2011 Apop.pdf - Lab-JOT

XP Monoclonal Antibodies
for Apoptosis and Autophagy
XP Monoclonal Antibodies are a line of high quality
rabbit monoclonal antibodies exclusively available
from Cell Signaling Technology (CST). Any product
labeled with XP has been carefully selected based on
superior performance in all approved applications.
XP Monoclonal Antibodies are generated using
XMT ® technology, a proprietary monoclonal method
developed at CST. This technology provides access to a
broad range of antibody-producing B cells unattainable
with traditional monoclonal technologies, allowing more
comprehensive screening and the identification of XP
monoclonal antibodies with:
eXceptional specificity
As with all CST antibodies, the antibody is specific to your
target of interest, saving you valuable time and resources.
+ eXceptional sensitivity
The antibody will provide a stronger signal for your target protein
in cells and tissues, allowing you to monitor expression of low
levels of endogenous proteins, saving you valuable materials.
+ eXceptional stability and reproducibility
XMT technology combined with our stringent quality control ensures
maximum lot-to-lot consistency and the most reproducible results.
= eXceptional Performance
XMT technology coupled with our extensive antibody validation and
stringent quality control delivers XP monoclonal antibodies with
eXceptional Performance in the widest range of applications.
LC3A (D50G8) XP Rabbit mAb #4599
is an example of an antibody with superior performance in a
wide range of tested applications.
Events
A
LC3A
C
LC3A (D50G8) XP Rabbit mAb #4599: Flow cytometric analysis of chloroquine-treated
HeLa cells (A) using #4599 (blue) compared to Rabbit (DA1E) mAb IgG XP Isotype Control
#3900 (red). IHC analysis of paraffin-embedded human glioblastoma multiforme (B) using
#4599. Confocal IF analysis of HeLa cells, untreated (C) or chloroquine-treated (D), using #4599
(green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5 ®
#4084 (fluorescent DNA dye).
B
D
Antibodies and Kits for the Study of
Apoptosis
and Autophagy
Cell Signaling Technology provides the highest possible quality
activation-state and total protein antibodies available for the study of
signaling pathways central to cell survival and programmed cell death.
CST antibodies have been extensively validated by our in-house
scientists in applications including western blotting, immunofluorescence,
flow cytometry, immunohistochemistry, chromatin immunoprecipitation
(ChIP), and ELISA. CST phosphorylation-specific
antibodies are the most highly cited and serve as core reagents in
multiple drug discovery platforms. Comprehensive and up-to-date
information can be found at our website.
The Bcl-2 family of proteins is composed of pro- and anti-apoptotic members that homo and heterodimerize
with one another. Together, these serve as a rheostat mechanism to regulate the onset of apoptosis at the
mitochondrion. In this image, the balance is in favor of the pro-apoptotic Bad proteins (red) that outnumber
the anti-apoptotic Bcl-xL proteins (green). As a result, the mitochondrial pore composed of VDAC in the
inner membrane and ANT in the outer membrane (both in brown) is shown releasing small molecules into
the cytosol during the permeability transition. Cytochrome c (blue) below the outer mitochondrial membrane
is poised to spill into the cytosol and trigger apoptosis once the outer mitochondrial membrane ruptures.
Table of Contents
4 Antibody Sampler Kits
5 Autophagy
6 Caspase Signaling
8 Cleaved Substrates
9 Antibody Validation
10 Bcl-2 Family Members
12 PathScan ® ELISA Kits and Antibody Pairs
13 Apoptosis Inhibitor Proteins / Mitochondrial Proteins
14 TNFR Family
15 Adaptor Proteins
16 NF-kB
18 p53 / Other Transcriptional Regulators
Other Signaling Proteins /
19 Granzymes and Other Proteases
20 Signaling Pathways
Visit our website for more experimental details, additional information,
and a complete list of available XP monoclonal antibodies.
© 2/2011 Cell Signaling Technology, Inc.
eXceptional Performance , Cell Signaling Technology ® , CST , PathScan ® , SignalSilence ® , XMT ® and XP are registered trademarks or trademarks of Cell Signaling Technology, Inc.
Selected rabbit monoclonal antibodies are produced under license (granting certain rights including those under U. S. Patents No. 5,675,063 and in some instances 7,429,487) from Environmental commitment: eco.cellsignal.com
Epitomics, Inc. Alexa Fluor ® and MitoTracker ® are registered trademark of Molecular Probes, Inc. / DRAQ5 ® is a registered trademark of Biostatus Limited. / Acumen ® is a registered
trademark of TTP Labtech.
PathScan ® Apoptosis and Proliferation MultiPlex IF Kit: Some kit components are provided under an agreement between Life Technologies Corporation and Cell Signaling Technology, Inc., and the manufacture, use, sale or import of antibody conjugate in this product is subject to one or more US patents and
corresponding non-US equivalents, owned or controlled by Life Technologies Corporation or its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether
the buyer is an academic or for-profit entity), for immunocytochemistry, high content screening (HCS) analysis, or flow cytometry applications. The sale of this product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic,
diagnostic or prophylactic purposes; (4) resale, whether or not such product or its components are resold for use in research; or for any other commercial purpose. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cellular
Analysis Business Unit, Busi ness Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
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