Significance, Detection and Elimination of ... - Minerva Biolabs

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The Usual Suspects • Contamination with other cell lines • Yeast • Fungi • Viruses: especially bovine Pestiviruses BVDV – Virus of Bovine Virus diarrhea CSFV – Virus of the classical swine but also BDV (Borna Disease Virus) • Bacteria • Mycoplasma -2-
Cross-contamination The contamination of a cell line with a cells from another type • Avoidance: Only one cell line under the clean bench at a time Separate medium bottles for each cell line • Detection: Difficult, especially with cell line of comparable morphology Indications are changes in growth rates, morphology of prduct characterisation as well as not reproducable results Applicable detection techniques: Isoenzyme pattern analysis, DNA sequencing, Southern blot bo analysis ayss -3-
Page 1: Significance, Detection and Elimina
Page 5 and 6: Yeast unicellular fungi size can va
Page 7 and 8: Fungi Medium color turns yellow qui
Page 9 and 10: Contamination with Pestivirus • B
Page 11 and 12: The Usual Suspects • Contaminatio
Page 13 and 14: Bacteria Contamination - Examples
Page 15 and 16: Analysis using Onar ® EUB 1 kb DNA
Page 17 and 18: Mycoplasma • Found in1898 classif
Page 19 and 20: Mycoplasma (continued) • Parasite
Page 21 and 22: Mycoplasma in the Electron Microsco
Page 23 and 24: Effects on Cell Culture • Inhibit
Page 25 and 26: Frequency and Source of Mycoplasma
Page 27 and 28: Sources of Contamination (continued
Page 29 and 30: Instruction for Testing • Regulat
Page 31 and 32: Culture method • Strict requireme
Page 33 and 34: Alternative Mycoplasma Diagnostic M
Page 35 and 36: Performance Sample DNA-Extraction P
Page 37 and 38: Preparation of the Venor ® GeM Kit
Page 39 and 40: Features of Venor ® GeM • Valida
Page 41 and 42: Frequency of Testing • New cell a
Page 43 and 44: Mycoplasma Elimination Methods •
Page 45 and 46: Effect of Mynox ® on Mycoplasma El

Significance, Detection and Elimination of ... - Minerva Biolabs

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