Significance, Detection and Elimination of ... - Minerva Biolabs

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PCR Mastermix • The correct order 1. Water 2. Buffer 3. Other components • Important! • Do not vortex Taq polymerase • Mix your reaction samples • Aliquot and then add your sample • Mix and centrifuge -38-
Features of Venor ® GeM • Validated according to Eur. Ph. 2.6.7, 5.8 • Recommended by the WHO • More than 100x cited in publications • Specific for > 25 mycoplasma species • Detection limit 1,5 copies/µL, LOD 95% = 4,5 copies/µl • Clear yes/no-result after 3 hours • Package sizes: 25, 50, 100 und 250 tests, t • User friendly aliquotes á 25 tests. • Aliquots of Master-Mix can be stored frozen including hot-start Taq possible. • Also available for real-time PCR -39-
Page 1 and 2: Significance, Detection and Elimina
Page 3 and 4: Cross-contamination The contaminati
Page 5 and 6: Yeast unicellular fungi size can va
Page 7 and 8: Fungi Medium color turns yellow qui
Page 9 and 10: Contamination with Pestivirus • B
Page 11 and 12: The Usual Suspects • Contaminatio
Page 13 and 14: Bacteria Contamination - Examples
Page 15 and 16: Analysis using Onar ® EUB 1 kb DNA
Page 17 and 18: Mycoplasma • Found in1898 classif
Page 19 and 20: Mycoplasma (continued) • Parasite
Page 21 and 22: Mycoplasma in the Electron Microsco
Page 23 and 24: Effects on Cell Culture • Inhibit
Page 25 and 26: Frequency and Source of Mycoplasma
Page 27 and 28: Sources of Contamination (continued
Page 29 and 30: Instruction for Testing • Regulat
Page 31 and 32: Culture method • Strict requireme
Page 33 and 34: Alternative Mycoplasma Diagnostic M
Page 35 and 36: Performance Sample DNA-Extraction P
Page 37: Preparation of the Venor ® GeM Kit
Page 41 and 42: Frequency of Testing • New cell a
Page 43 and 44: Mycoplasma Elimination Methods •
Page 45 and 46: Effect of Mynox ® on Mycoplasma El

Significance, Detection and Elimination of ... - Minerva Biolabs

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